CuSO_4和烯效唑对寒地粳稻成熟胚培养的影响  

Effect of CuSO_4 and Uniconazole on Culture of Mature Embryos of Japonica Rice

作　　者：山禾 邹德堂[1] 萨日娜[2] 张皓政[1] 

机构地区：[1]东北农业大学水稻研究所,哈尔滨150030 [2]黑龙江省农业科学院南繁基地,哈尔滨150086

出　　处：《吉林农业科学》2015年第1期19-22,28,共5页Journal of　Jilin Agricultural Sciences

基　　金："十二五"农村领域国家科技计划课题(2013BAD20B04);科技部科技攻关项目(2011BAD35B02-01);科技部科技支撑项目(2011BAD16B11)

摘　　要：为了建立寒地粳稻成熟胚的高频再生体系,以寒地粳稻空育131和东农425为试验材料进行成熟胚培养,研究CuSO4和烯效唑的单独和组合使用对水稻成熟胚培养的影响。结果表明:在培养基中单独添加15.0μmol/L的Cu2+或添加0.50 mg/L的烯效唑有助于寒地粳稻成熟胚的诱导继代和分化;在诱导和分化培养基中同时添加CuSO4和烯效唑时,空育131在CuSO4浓度为20.0μmol/L且烯效唑浓度为1.00 mg/L时,愈伤组织的诱导率和分化率都达到最高;CuSO4浓度为10.0μmol/L,烯效唑浓度为1.00 mg/L时,东农425愈伤组织的诱导率最高,CuSO4浓度为15.0μmol/L,烯效唑浓度为1.00 mg/L时,东农425愈伤组织的分化率最高。In order to establish the system of high frequency plant regeneration for japonica rice mature embry-os, effects of different concentrations of CuSO4 and Uniconazole on in vitro culture of mature embryos were studiedusing two rice cultivars of Kongyu 131 and Dongnong 425. The results showed that callus induction and plantlet dif-ferentiation rate of japonica rice mature embryos were apparently improved on the medium with 15.0 μmol/L CuSO4 or 0.50 mg/L Uniconazole. When 20.0 μmol/L CuSO4 and 1.00 mg/L Uniconazole were added simultaneously in theinduction and differentiation medium,the callus induction and plantlet differentiation rate of Kongyu 131 was thehighest.When the concentration of CuSO4 was 10.0 μmol/L and concentration of Uniconazole was 1.00 mg/L, the cal-lus induction rate of Dongnong 425 was the highest. For the plantlet differentiation rate of Dongnong 425, the idealconcentrations of CuSO4 and Uniconazole were 15.0 μmol/L and 1.00 mg/L.

关 键 词：寒地粳稻 成熟胚 组织培养 CUSO4 烯效唑 

分 类 号：S511[农业科学—作物学]