鸦胆子油乳制剂对宫颈癌Hela细胞的抑制效果及作用机制研究  

Study on the inhibition effect and the mechanism of brucea javanica oil emulsion preparation in cervical cancer Hela cells

作　　者：罗琦[1] 杨静[1] 

机构地区：[1]武汉大学基础医学院,湖北武汉430000

出　　处：《现代中西医结合杂志》2015年第6期574-576,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨鸦胆子油乳制剂对宫颈癌Hela细胞的抑制效果及作用机制。方法观察不同浓度（5，10μg／mL）鸦胆子油乳处理Hela细胞后的形态改变；检澳l不同浓度鸦胆子油乳（2．5，5，10，20，40μg／mL）处理后对Hela细胞的抑制率，并观察鸦胆子油乳处理浓度分别为10，20μg／mL时细胞周期的变化情况。结果鸦胆子油乳处理宫颈癌Hela细胞后出现凋亡小体，且细胞毒作用随时间延长毒性增强；在处理48h后，研究组的抑制率均〉60％，72h后抑制率均〉80％；伴随鸦胆子油乳浓度的增加，G。～G1期细胞比率降低，S期及G2～M期细胞比率明显增加。10μg／mL和20μg／mL鸦胆子油乳处理48h后细胞凋亡率分别为60％与70％。结论鸦胆子油乳能够明显抑制宫颈癌Hela细胞的增殖，且呈时间依赖性，能够诱导细胞凋亡，且使Hela细胞阻滞于S期。Objective It is to observe the inhibitory effect of Brucea javanica oil emulsion preparation on cervical cancer Hela cells, and approach its mechanism. Methods The morphology change of Hela cell after treated with different concentra- tions （5, l0μg/mL） of brucea javanica oil was observed. The inhibition rate of Hela cells after treatment by different concen- trations （2. 5, 5, 10, 20, 40μg/mL） of brucea javanica oil emulsion on Hela cell were detected, and the change of cell cy- cle of Hela cell treated by 10 and 20μg/mL of brueea javaniea oil emulsion was observed. Results Apoptotic bodies was founder after treatment of brucea javanica oil emulsion in cervical cancer Hela cells, and the cytotoxic effect was increased with prolonged time; each group had more than 48% of inhibition rate after treated 48-hour, , and more than 80% after 72-hour; with the increase of brueea javaniea oil emulsion concentration, the ratio of Go to G1 phase cells was decreased, S phase and G2 to M phase cell ratio were increased significantly. The cell apoptosis rate after treated with 10 and 20μg/mL of brucea ja- vanica oil emulsion for 48-hour were 60% and 70%. Conclusion Brucea javanica oil emulsion can obviously inhibit the prolif- eration of cervical cancer Hela cells, and was time dependent, it can induce cell apoptosis, and arrest the Hela cell cycle in S phase.

关 键 词：宫颈癌HELA细胞 鸦胆子油乳 抑制增殖 巴氏染色 细胞凋亡 

分 类 号：R965[医药卫生—药理学]