血红蛋白F与血红蛋白A2之间的交叉互作  

Cross Interaction Between Hb F and Hb A2

作　　者：韩丽红[1] 杨文杰[1] 高丽君[1] 苏燕[1] 秦文斌[1] 

机构地区：[1]包头医学院血红蛋白研究室,内蒙古包头014010

出　　处：《中国医药指南》2015年第5期62-63,共2页Guide of China Medicine

摘　　要：目的我们曾经用淀粉-琼脂混合凝胶电泳证明成人血红蛋白A1与A2发生交叉互作,当时未对胎儿血红蛋白F进行研究。现在,再用这种方法研究血红蛋白F与血红蛋白A2是否也有相互作用。方法由脐带血分离红细胞,再用淀粉-琼脂糖混合凝胶电泳制备纯的血红蛋白F,然后让血红蛋白F穿过成人红细胞溶血液,观察穿过溶血液后血红蛋白A2区带变形情况。结果血红蛋白F穿过成人红细胞溶血液中的血红蛋白A2时,后者变形,与游离血红蛋白F相比,进入溶血液中的血红蛋白F泳速稍慢。结论血红蛋白F与血红蛋白A2也能发生交叉互作。Objective We have demonstrate that adult hemoglobin Hb A1 and Hb A2 can cross interact each other by starch-agarose mixed gel electrophoresis,but the fetal hemoglobin Hb F was not studied at that time.At present,we use this method to study whether the interaction between Hb F and Hb A2 occur.Methods Separating red blood cells from the umbilical cord,then preparation pure hemoglobin F from the starch-agarose electrophoresis gel electrophoresis,and let hemoglobin F dissolve blood through adult red blood cells was observed after dissolving blood hemoglobin A2 through the deformation zone.Results The dissolution of erythrocyte hemoglobin F in the blood through the adult hemoglobin A2,which is deformed as compared with the free hemoglobin F,hemoglobin in the blood solution into the F swimming speed slower.Conclusion The results showed that the cross interaction was occurred as well between Hb F and Hb A2.

关 键 词：淀琼电泳 胎儿血红蛋白 血红蛋白F 血红蛋白A2 交叉互作 

分 类 号：R392.12[医药卫生—免疫学]