高效液相色谱法测定丹参胶囊中8种有效成分的含量  

HPLC determination of eight active constituents in Danshen capsules

作　　者：张爱兵 张珺[2] 程月发[2] 裴璐 

机构地区：[1]河北省唐山市食品药品检验中心,河北唐山063000 [2]河北联合大学冀唐学院,河北唐山063300

出　　处：《中南药学》2015年第1期80-82,共3页Central South Pharmacy

基　　金：唐山市2013年科技支撑计划项目(No.13130298z);2012年河北联合大学科学研究基金项目(No.z201242)

摘　　要：目的采用高效液相色谱法同时测定丹参胶囊中丹参素、原儿茶醛、迷迭香酸、丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量。方法采用Agilent Eclipse XDB-C18（250 mm×4.6 mm,5μm）色谱柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速1 m L·min-1,检测波长为280 nm。结果丹参素、原儿茶醛、迷迭香酸、丹酚酸B、二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA分别在0.120~2.40μg（r=0.996 7）、0.174~3.480μg（r=0.999 9）、0.176~3.52μg（r=0.999 9）、0.802~16.0μg（r=0.998 6）、0.080 0~1.60μg（r=0.999 9）、0.162~3.24μg（r=0.999 9）、0.260~5.20μg（r=0.999 9）和0.176~3.52μg（r=0.999 9）与峰面积呈良好的线性关系;加样回收率在97.8%~99.4%。结论该方法稳定性好,重复性好,可用于丹参胶囊质量的控制。Objective To establish an HPLC method to determine the content of danshensu, protocatechuic aldehyde, rosmarinc acid, salvianolic acid B, dihydrotanshione Ⅰ, croptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ A simultaneously in Danshen capsules. Methods The determination was performed on an Agilent Eclipse XDB-C18column（250 mm×4.6 mm, 5 μm） by gradient elution using 0.1%（v/v） phosphoric acid-acetonitrile as the mobile phase, the flow rate was 1 m L · min- 1, and the detection wavelength was 280 nm. Results There were good linearity between 8 components of peak areas and the ranges of 0.120- 2.40 μg（r = 0.996 7） for danshensu, 0.174- 3.480μg（r = 0.999 9） for protocatechuic aldehyde, 0.176- 3.52 μg（r = 0.999 9） for rosmarinc acid, 0.802- 16.0 μg（r = 0.998 6） for salvianolic acid B, 0.080 0- 1.60 μg（r = 0.999 9） for dihydrotanshione Ⅰ, 0.162- 3.24 μg（r =0.999 9） for croptotanshinone, 0.260- 5.20 μg（r = 0.999 9） for tanshinone Ⅰ and 0.176- 3.52 μg（r = 0.999 9） for tanshinone Ⅱ A. The average recoveries of the method were between 97.8% and 99.4%. Conclusion The mehod is stable and reproducible, which can be used to control the quality of Danshen capsules.

关 键 词：丹参胶囊 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]