枯草芽孢杆菌21抑菌物质的稳定性分析及初步分离  

Stability Analyzing and Component Isolating of Antagonistic Substanc Eproduced by Bacillus subtilis Strain 21

作　　者：郭春兰[1] 于春生[1] 张雨竹[1] 宋志儒 杨月[1] 肖亚静[1] 孙冬梅[1] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2015年第1期69-72,共4页journal of heilongjiang bayi agricultural university

基　　金：黑龙江八一农垦大学研究生创新科研项目(YJSCX2013-23BYND)

摘　　要：为了探讨芽孢杆菌21抑菌物质的稳定性及确定其抑菌主要成分,利用管碟法和硫酸铵沉淀法研究了代谢物对玉米圆斑病菌抑菌能力的变化。结果表明：经p H 4-9处理后,代谢产物对玉米圆斑病菌的抑制能力在60%-100%之间变化;经碱性蛋白酶和木瓜蛋白酶处理后,代谢产物对玉米圆斑病菌的抑制能力在70%-80%之间;但经胰蛋白酶和蛋白酶K处理后,抑菌能力变化不大,仍在95%以上;经40-90℃处理后,抑菌能力变化范围在67%-100%之间,随温度升高,抑菌能力有下降趋势,但当处理温度为100℃时,发酵液几乎没有抑菌效果;代谢物经30%-70%不同浓度的饱和硫酸铵沉淀后,蛋白类沉淀物均具有抑菌作用,其中以饱和度为60%的硫酸铵沉淀后的抑菌效果最好,抑菌半径可达1.0 cm。To explore the stability of antimicrobial ingredients of Bacillus subtilis 21 and to define the main components of fermentation,the cylinder-plate method and the ammonium sulfate precipitation method were used to study the inhibitory change of liquid broth fermentation to Bipolaris carbonum. The result showed that the inhibitory activity was retained from 60% to 100% after p H 4-9 treatment;the inhibitory activity was retained from 70% to 80% after by alkaline protease and papain treatment,but the inhibitory activity was retained over 95% after by trypsin and proteinase K treatment;the inhibitory activity changed from 67% to100% after 40-90 ℃ treatment and the inhibitory activity was decreased as the temperature increasing,and the inhibitory activity disappeared when treatment temperature was at 100 ℃,Protein precipitates with 30% to 70% ammonium sulfate saturated strongly inhibited the mycelia growth of B. carbonum,in which the optimal concentration of ammonium sulfate was 60%,and the inhibition zone diameter was up to 1.0 cm.

关 键 词：枯草芽孢杆菌 抑菌物质 稳定性分析 粗提物 

分 类 号：Q939.9[生物学—微生物学]