人工组合纤维素分解复合菌系的构建及各组分对纤维素分解的影响  

Construction of Cellulose Decomposing Complex Microbial System and Effects of Different Component on Cellulose Degradation

作　　者：李振国[1] 温雪[1] 张杨[1] 高亚梅[1] 晏磊[1] 王彦杰[1] 王伟东[1] 包军[2] 

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319 [2]东北农业大学动物科技学院

出　　处：《黑龙江八一农垦大学学报》2015年第1期78-84,共7页journal of heilongjiang bayi agricultural university

基　　金："十二五"国家科技支撑计划项目(2012BAD12B05);黑龙江省杰出青年科学基金项目(JC201002);黑龙江省科技攻关项目(GC12B306);黑龙江省高校科技创新团队项目(2012TD006)

摘　　要：为研究复合菌系高效协同分解纤维素的机制,从天然富集且高效稳定分解纤维素的复合菌系WSC-9中分离获得的8株细菌纯培养进行组合,构建由3株厌氧菌(W-A、W-B、W-C)和2株好氧菌(W-3、W-5)组成且具有较高纤维素分解能力的组合,PCS培养基中50℃静止培养10 d可分解97.6%的滤纸。组合减少试验显示,W-B为纤维素分解的关键菌株,减少组合中任一菌株均会降低滤纸分解率,培养基中代谢产物乙醇、乙酸、丙酸等含量也发生变化。W-3和W-5对厌氧菌分解滤纸具有辅助作用,W-B和W-C分别对滤纸分解初期的乙醇和后期的己酸含量有重要影响,厌氧菌对培养基p H均有调节作用。To study the mechanism of cellulose degradation by composite of microbial community,the artificial microbial system with capable of cellulose degradation was constructed. The community consisted with three strains of anaerobic bacteria（W-A,W-B and W-C）and two strains of aerobic bacteria（W-3 and W-5）from eight pure culture strains that isolated from microbial community of WSC-9. The results showed that 97.6% of filter paper was decomposed after 10 days culture in PCS medium by the microbial system under static condition of 50 ℃. The reduce test showed that W-B was the key strain for cellulose degradation,the degradation rates of filter paper would be decreased when any strain was reduced,and the products of the microbial system such as ethanol,acetic,and propionic acid were also changed. The aerobic isolates of W-3 and W-5 were the important and necessary for filter paper degradation performed by anaerobic isolates. W-B and W-C also had the important effect on the content of ethanol in initial stage and caproic acid in later stage of filter paper degradation. The anaerobic isolates also had the certain regulatory effect on the culture environment p H.

关 键 词：纤维素分解 复合菌系 组合 分解率 代谢产物 

分 类 号：Q939[生物学—微生物学]