microRNA-155对于人膀胱癌细胞um-uc-3的迁移和侵袭能力影响的研究  

The migration and invasion promoting effects of micro RNA-155 on bladder cancer cell line of um-uc-3

作　　者：彭杨[1] 钟广正 董文[1] 林天歆[1] 

机构地区：[1]中山大学孙逸仙纪念医院泌尿外科,广州510120

出　　处：《岭南现代临床外科》2015年第1期1-5,共5页Lingnan Modern Clinics in Surgery

基　　金：国家自然科学基金(81101935);国家自然科学基金(81272808);广东联合基金项目(U1301221)

摘　　要：目的前期研究发现mi R-155在多种实体瘤中具有促肿瘤细胞增殖的作用,本研究探讨mi R-155对人膀胱癌细胞um-uc-3转移相关的生物学行为的影响。方法 (1)通过组织RNA研究,来讨论mi R-155在膀胱癌组织中的表达差异;(2)将人工合成mi R-155-mimics转入人膀胱癌细胞um-uc-3,并设立对照组;(3)通过q RT-PCR检测mi R-155的转染效率;(4)划痕试验研究过表达mi R-155的um-uc-3细胞迁移能力变化;(5)Transwell试验检测mi R-155对um-uc-3细胞迁移、侵袭的影响;(6)Western-blot及q PCR检测肿瘤转移相关基因的表达情况。结果 (1)组织中,q RT-PCR证实mi R-155的表达量在膀胱癌组织中较癌旁组织增高;(2)q RT-PCR检测mi R-155转染效率,实验组表达量明显增高(P<0.01);(3)划痕实验证实mi R-155过表达的um-uc-3细胞有更强的迁移能力;(4)Transwell试验发现mi R-155可以促进um-uc-3细胞迁移、侵袭,与对照组差异显著(P<0.01);(5)过表达mi R-155的um-uc-3细胞中,β-catenin、vimentin、snail的表达量在RNA水平和蛋白水平都比对照组显著增高,但不涉及claudin-1。结论 mi R-155可以促进人膀胱癌细胞um-uc-3的迁移和侵袭。Objective To study the effects of micro RNA-155（miR-155） on migration and invasion of bladder cancer cell um-uc-3. Methods Mi R-155 expression was detected in bladder cancer tissues. After exogenous transfection of miR-155 mimics into um-uc-3,.Transwell chamber assay and wound healing assay were performed to assess cell migration and invasion..Expression of EMT markers was evaluated by quantitative real-time PCR and western blotting. Results Mi R-155 expression was higher in bladder cancer tissues than adjacent normal tissues.Over-expression of miR-155 promoted migration and invasion in bladder cancer cells of um-uc-3..Both m RNA and protein expressions of β-catenin, vimentin and snail were higher in miR-155 transfected cells, while claudin-1 showed no significance. Conclusion Over-expression of miR-155 can promote migration and invasion of bladder cancer cells with higher expressed EMT markers like β-catenin, vimentin and snail.

关 键 词：膀胱癌 微小RNA 迁移 侵袭 EMT 

分 类 号：R694[医药卫生—泌尿科学] R737.14[医药卫生—外科学]