血竭壳聚糖药膜对大鼠糖尿病创面Wnt/β-catenin信号的影响  

Effect of Xuejie-Chitosan membrane on Wnt / β-catenin pathway in a model of chronic wound in diabetic rats

作　　者：朱颉[1] 曹亿[1] 于萌蕾[1] 罗自通[1] 彭吉才[1] 黄都平 曾兵[1] 

机构地区：[1]中山大学孙逸仙纪念医院南院急诊,广州510120

出　　处：《岭南现代临床外科》2015年第1期99-102,共4页Lingnan Modern Clinics in Surgery

基　　金：吴阶平医学基金(320.6750.14139)

摘　　要：目的观察血竭壳聚糖药膜对糖尿病大鼠创伤愈合和创面组织Wnt/β-catenin信号通路的影响。方法采用链脲佐菌素(STZ)注射法建立大鼠糖尿病创面模型,实验组给与血竭壳聚糖药膜治疗,对照组采用常规换药治疗,观察两组创面愈合等情况。采用Western blot及q RT-PCR检测创面β-catenin、VEGF及Cyclin D1蛋白和m RNA的表达。结果血竭壳聚糖药膜能够明显促进糖尿病大鼠创伤的愈合,实验组创面愈合情况较对照组显著提高,差异具有统计学意义(P<0.01)。实验组创面组织β-catenin、VEGF及Cyclin D1蛋白和m RNA的表达较对照组明显增加(P<0.01)。结论血竭壳聚糖药膜治疗大鼠糖尿病创面效果良好,其机制可能与活化Wnt/β-catenin信号有关。Objective To evaluate the effect of Xuejie-Chitosan membrane on diabetic chronic wound of experimental rats and the expression of Wnt / β-catenin pathway. Methods Diabetic chronic wound model was made by intraperitoneal injection of streptozocin. The experimental group was treated with Xuejie-Chitosan membrane, and the control group was treated with conventional treatment.Western blot and q RT-PCR were used to determine the expression of β-catenin, VEGF and Cyclin D1.Wound healing area was calculated by image-proplus 6.0. Results The wound healing rate in experimental group was significantly higher than in control group（P〈0.01）. Western blot and q RTPCR results showed that both the m RNA and protein levels of β-catenin, VEGF and Cyclin D1 were up-regulated when compared to the control group（P〈0.01）. Conclusion Xuejie-Chitosan membrane is superior to conventional treatment on chronic wound in diabetic rats. The activation of Wnt / β-catenin signals may help to promote healing of diabetic chronic wound.

关 键 词：祛腐生肌 Wnt/β-catenin信号 糖尿病创面 

分 类 号：R641[医药卫生—外科学]