骨髓间充质干细胞对环磷酰胺诱导卵巢损伤的保护作用  

Protective Effect of Bone Marrow Mesenchymal Stem Cells Transplantation on Cyclophosphamide-Induced Ovarian Damage in Rat Model

作　　者：左侠[1,2] 张坤[1,2] 王懿[3] 王明星[4] 陈蕾[5] 

机构地区：[1]第四军医大学 [2]西安市第四医院 [3]长安大学数学系 [4]中国人民解放军96215部队卫生队 [5]中国人民解放军海军总医院妇产科

出　　处：《现代生物医学进展》2014年第34期6629-6633,共5页Progress in Modern Biomedicine

基　　金：军队十二五科技攻关项目(CHJ11J105)

摘　　要：目的:研究骨髓间充质干细胞(MBSc)对注射环磷酰胺(CTX)引起的大鼠卵巢损伤的保护作用。方法:将30只SD大鼠按随机数字表随机分为三组,即对照组,模型组及细胞移植组,分别经尾静脉接受生理盐水,CTX和CTX+MBSc移植。监测大鼠体重,动情周期的变化,用药结束后一周处死大鼠,测定血清雌二醇(E2),卵泡刺激素(FSH),黄体生成素(LH)的变化,观察各组大鼠卵巢形态学及卵泡数量变化,并利用原位细胞凋亡TUNEL法检测3组大鼠卵巢中细胞凋亡指数(AI)。结果:与对照组比较,模型组大鼠体重下降,动情周期延长,卵泡数量下降,E2下降,FSH及LH升高,组织细胞AI增加。与模型组比较,细胞移植组大鼠体重增加,动情周期缩短,卵泡数量增加,尤其是中大卵泡数增加,E2上升,LH下降,FSH接近正常。结论:MBSc移植能通过减少卵巢组织AI而在一定程度上减少环磷酰胺引起的卵巢功能损伤。Objective： To study the protective effects of bone marrow mesenchymal stem cells（MBSc） on cyclophosphamide（CTX）-induced ovarian injury in female rats. Methods： 30 SD rats were randomly divided into three groups（Control,Model,Trans）with random number table, which were treated by tail vein injection with normal saline, cyclophosphamide（CTX） and MBSc transplantation following CTX injection respectively. We observed body weights and estrous cycles of all rats during the experiment and harvested to check the content changes of serum concentration of estradiol（E2）, follicle-stimulating hormone（FSH） and luteinizing hormone（LH）,and observed the histological and pathological changes, the follicle number and apoptosis of ovarian tissue on 7th day after drug withdrawal. Results：（1） The follicle number and serum concentration of E2 in rats of the Model group were lower than Control group,while the serum concentration of FSH and LH and AI in rats of the Model group were higher than Control group.（2） The body weight,the follicle number and serum concentration of E2 in rats of the Trans group were higher than Model group, while the serum concentration of FSH and LH and AI of rats in Trans group were lower than Model group. Conclusions： Transplantation of MBSc could reduce CTX-included injury of ovarian function by reducing AI of ovarian tissue.

关 键 词：骨髓间充质干细胞 环磷酰胺 卵巢损伤 卵巢早衰 

分 类 号：Q953[生物学—动物学] R711.75[医药卫生—妇产科学]