抗核抗体谱十八项检测在系统性红斑狼疮中的临床应用  

The Clinical Application of Detection of Antinuclear Antibodies Spectrum Eighteen Items in Systemic Lupus Erythematosus

作　　者：罗秀霞[1,2] 尹志华[1,2] 曹智君[1,2] 陈杰[1,2] 叶志中[1,2] 

机构地区：[1]广东医学院附属福田医院香蜜湖风湿病分院 [2]广东医学院风湿病研究所,广东深圳518040

出　　处：《现代生物医学进展》2014年第34期6676-6679,共4页Progress in Modern Biomedicine

基　　金：国家高技术研究发展计划(863计划)课题资助项目(012AA02A513);深圳市医学重点学科建设资助项目(2005C10);深圳市福田区科技局课题资助项目(FTWS089)

摘　　要：目的:采用抗核抗体谱(ANA谱)十八项检测系统性红斑狼疮(SLE)患者,探讨其在SLE诊治中的临床应用价值。方法:选择2011年2月至2012年12月我院风湿科收治的370例患者,其中系统性红斑狼疮(SLE)患者158例、其他自身免疫性疾病患者142例,其他疾病患者70例,均采用间接免疫荧光法和免疫印迹法检测其ANA谱十八项,并对结果进行回顾性分析。结果:SLE患者ANA谱十八项中ANA(96.84%、55.47%、27.10%,P<0.01)、Anti-SSA/Ro60(55.06%、14.34%、3.39%,P<0.01)、Anti-SSA/Ro52(46.20%、12.83%、1.69%,P<0.01)、Anti-Sm D1(31.65%、2.64%、1.69%,P<0.01)、Anti-U1-sn RNP(25.95%、2.26%、0%,P<0.01)、Anu A(25.32%、0.75%、0%,P<0.01)、Anti-SSB/La(25.32%、5.66%、0.85%,P<0.01)、Anti-ds DNA(24.05%、0.38%、0%,P<0.01)、AHA(20.89%、0.38%、0%,P<0.01)、Anti-P0(18.35%、0.38%、0%,P<0.01)的阳性率均显著高于其他自身免疫性疾病患者和其他疾病患者。96.84%SLE患者被检测出ANA阳性,以颗粒型为主(占51.63%)。81.65%的SLE患者一次性检出2项及以上抗体。结论:ANA谱十八项检测有助于系统性红斑狼疮的筛查和诊断。Objective： To explore the clinical application value of detection of antinuclear antibodies（ANA） spectrum eighteen items in the diagnosis and treatment of systemic lupus erythematosus（SLE）. Methods： Three hundred seventy inpatients admitted in the rheumatology department of our hospital from February 2011 to December 2012 were selected, including SLE patients（158 cases）, other autoimmune diseases patients（142 cases） and other diseases patients（70 cases）. The ANA spectrum eighteen items were detected with indirect immunoflurescence and immunoblotting methods. The results of ANA spectrum were retrospectively analyzed. Results： The positive rate of ten antibodies of ANA spectrum eighteen items showed significant defference between SLE group, other autoimmune diseases group and other disease group, including ANA（96.84%, 55.47%, 27.10%, P0.01）, Anti-SSA/Ro60（55.06%, 14.34%, 3.39%,P0.01）, Anti-SSA/Ro52（46.20%, 12.83%, 1.69%, P0.01）, Anti-Sm D1（31.65%, 2.64%, 1.69%, P0.01）, Anti- U1-sn RNP（25.95%,2.26%, 0%, P0.01）, Anu A（25.32%, 0.75%, 0%, P0.01）, Anti- SSB/La（25.32%, 5.66%, 0.85%, P0.01）, Anti-ds DNA（24.05%, 0.38%,0%, P0.01）, AHA（20.89%, 0.38%, 0%, P0.01）, and Anti-P0（18.35%, 0.38%, 0%, P0.01）. The positive ANA were detected in 96.84%SLE patients and mainly with particle type（51.63%）. Two or more antibodies were detected positive in 81.65% SLE patients at the same time. Conclusion： The detection of ANA spectrum eighteen items was valuable for the screening and diagosis of SLE.

关 键 词：系统性红斑狼疮 抗核抗体谱 抗双链DNA抗体 抗核小体抗体 抗Sm D1抗体 

分 类 号：R593.24[医药卫生—内科学]