轴向量化载荷模拟＋Gz致兔腰椎间盘退变的实验研究  

Research on positive acceleration induced rabbit lumbar disc degeneration by means of quantized axial loading device

作　　者：王琦[1] 张载清 李广 虞攀峰[1] 刘斯瑶[1] 王建昌[1] 

机构地区：[1]空军总医院,北京100142 [2]空军军训器材研究所,北京100097

出　　处：《中华航空航天医学杂志》2014年第3期189-193,F0003,共6页Chinese Journal of Aerospace Medicine

基　　金：全军后勤科研“十二五”重大项目（AKJ11J004）

摘　　要：目的 观察可控轴向量化机械载荷模拟正加速度（＋Gz）重复暴露诱导的椎间盘退变动物模型病理变化,为＋Gz致飞行员腰椎间盘退变研究提供实验依据. 方法 将24只新西兰大白兔按照随机数字表法分为对照组、4周组和8周组,每组8只.实验组采用自行设计的可控轴向、量化加压装置,对兔腰4-s椎间盘直接施加40 N的压力,每日10次,每次15s,间隔5 min,4周组加压4周,8周组加压8周,对照组不加压.用椎间盘高度指数（disc height index,DHI）、MRI、组织学及免疫组织化学染色评价兔椎间盘加压4周和8周的退变程度. 结果 ①实验8周组和对照组、实验4周组比较,DHI百分率差异均有统计学意义（P＜0.01）,实验4周组与对照组比较差异无统计学意义（P＞0.05）;②实验8周组和对照组、实验4周组比较,MRI Pfirrmann分级差异均有统计学意义（Z=3.655、3.330,P＜0.01）,实验4周组与对照组比较差异无统计学意义（P＞0.05）;③实验8周组和对照组、实验4周组比较,组织学Thompson分级差异均有统计学意义（Z=2.893、2.095,P＜0.05）,实验4周组与对照组比较差异无统计学意义（P＞0.05）;④免疫组织化学示：实验8周组Ⅱ型胶原退变显著,Ⅰ型胶原在髓核中分布增加. 结论 DHI、MRI和病理学结果显示,实验组腰椎间盘较早发生退变,且受压时间越长,退变越显著.为飞行员腰椎间盘退变的进一步研究提供了实验依据.Objective To estimate the influence of simulated positive acceleration on rabbit lumbar disc degeneration by means of a controllable axial loading device,and to provide experimental reference for the further study on pilot＆#39;s lumbar disc degeneration.Methods Twenty four New Zealand white rabbits were randomly divided into 4-week group （n=8）,8 week group （n=8） and control group （n =8）.A self-designed controllable axial loading device was utilized to impose 40 N compress forces on the discs of the rabbits for 15 s,10 times a day for 4 and 8 weeks in two experimental groups respectively but in control group.Degeneration of the discs was evaluated by disc height index （DHI）,magnetic resonance imaging （MRI） scanning results,type Ⅰ and type Ⅱ collagen that got from haematoxylin and eosin （HE） staining,and immunohistochemical staining.Results ① Compared with that of control group and 4-week group,DHI of 8-week group had statistical difference （P〈 0.01）.There was no DHI difference between 4-week group and control group （P〉0.05）.②Compared with that of control group and 4 week group,Pfirrmann＆#39;s system （MRI） of 8 week group showed statistical difference （Z=3.655,3.330,P〈0.01）,but the difference between 4-week group and control group （P〉0.05）.③Compared with that of control group and 4-week group,Thompson grade of 8-week group showed statistical difference （Z=2.893,2.095,P〈0.05）.Thompson grade did not significantly differ between 4-week group and control group （P〉0.05）.④Immunohistochemical staining showed that the degeneration of type Ⅱ collagen in 8-week group was more serious.Staining for type Ⅰ collagen showed that the distribution of positive staining increased in nucleus pulposus.Conclusions According to DHI,MRI and pathology results,the lumbar disc of the rabbits in experimental group appear early degeneration,and become more serious as the loading time prolonged.It provides a good experimental reference for the further study of p

关 键 词：兔 椎间盘 磁共振成像 加速度 模型 动物 

分 类 号：R85[医药卫生—航空、航天与航海医学]