微泵驱动的微流控芯片流动注射气体扩散分离系统的研究(英文)  

Microfluidic Chip-Based Flow Injection System of Gas Diffusion Separation with Micropump Drive

作　　者：贾宏新[1,2] 关艳霞[3] 徐章润[2] 王建华[2] 

机构地区：[1]辽宁省食品检验检测院,辽宁沈阳110051 [2]东北大学分析科学研究中心,辽宁沈阳110819 [3]沈阳工业大学信息科学与工程学院,辽宁沈阳110870

出　　处：《分析科学学报》2014年第5期644-650,共7页Journal of Analytical Science

基　　金：National Natural Science Foundations of China(No.20975019);The Fundamental Research Funds for the Central Universities(N110805001)

摘　　要：本文提出一种毛细-蒸发作用力微泵作为液流驱动力的微流控芯片流动注射气体扩散分离检测系统,该系统可以连续、选择性地检测溶液中的NH+4。微流控芯片为六层结构,包含五层聚二甲基硅氧烷(PDMS)及一层玻璃。采用PDMS加工透气膜,并与其它PDMS层和玻璃层通过等离子体处理后封接。液芯波导管用作检测流通池,以提高检测光程。通过检测溶液中NH+4验证了微流控流动注射系统的性能。系统的检出限(3σ)为40μmol·L-1,分析通量可达60h-1,试样消耗仅100nL。A microfluidic chip-based flow injection system driven by micropumps based on capillary and evaporation effects for performing on-chip gas diffusion separation was proposed for the continuous and highly selective determination of ammonium.The microfluidic chip presented here consists of six layers,including five layers of poly（dimethylsiloxane）（PDMS）and one layer of glass.One of the PDMS layers used as the gas permeable membrane was tightly bonded with another PDMS layer and the glass layer by plasma treatment.A liquid-core waveguide（LCW）capillary was used as detection flow cell to increase the optical path length of absorbance detection.The whole system featured small scale and low cost.The performance of the resulting microfluidic chip-based flow injection system was demonstrated in the determination of ammonium ion.The limit of detection was 40μmol·L-1（3σ）,and the sample throughput was 60h-1with sample consumption of 100 nL for each cycle.

关 键 词：微流控芯片 气体扩散分离 流动注射 NH+4 微泵 

分 类 号：O657[理学—分析化学]