纤维素酶基因表达研究进展  

Research Progress in the Expression of Cellulase

作　　者：何敏超[1] 许敬亮[1] 袁振宏[1] 孔晓英[1] 张宇[1] 陈小燕[1] 梁翠谊[1] 

机构地区：[1]中国科学院广州能源研究所,中国科学院可再生能源重点实验室,广东广州510640

出　　处：《林产化学与工业》2014年第5期169-174,共6页Chemistry and Industry of Forest Products

基　　金：国家863计划资助(2013AA065803);国家自然科学基金资助项目(21176237);广州市科技攻关项目(2013J4300026)

摘　　要：获得更高生产性能的菌株是降低纤维素酶在纤维乙醇、纸浆造纸、纺织等领域使用成本的主要解决途径。总结了影响宿主菌(里氏木霉和毕赤酵母)高效表达纤维素酶基因的多种因素,如里氏木霉转录调控、启动子优化、外源酶基因与毕赤酵母表达的适配性、启动子拷贝数及酶基因拷贝数等,以期为纤维素酶高产菌株的构建提供有益的参考。Acquisition of a more excellent producing strain of cellulase is the major approach to decrease the cost of application in the field of cellulosic ethanol,pulp and paper,textile and so on. In order to review some valuable information on the construction of high yielding strain of cellulase,a variety of factors,including regulation of cellulase in Trichorderma reesei,optimization of promoter,the compatibility between cellulase gene and the host,the copy number of promoter and expressed gene,were discussed. Their influence on the expression of cellulse gene in T. reesei or Pichia pastoris was summarized.

关 键 词：纤维素酶 转录调控 基因表达 里氏木霉 毕赤酵母 

分 类 号：TQ35[化学工程]