黄药子醇提物肝细胞毒性机制的初步研究  

Preliminary study of the hepatotoxicity induced by Dioscorea bulbifera L. on hepatocytes

作　　者：李寅超[1] 石金金[1] 富显祖 何永侠[1] 孙曼[1] 陈慧芳[1] 吴翠萍[1] 

机构地区：[1]郑州大学药学院,河南郑州450001

出　　处：《时珍国医国药》2014年第10期2345-2346,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技部"十一五"科技支撑计划子课题(No.2006BAI06A03-03)

摘　　要：目的研究黄药子醇提物对肝HL-7702细胞生长的抑制作用及细胞乳酸脱氢酶释放率的影响。方法采用MTT法,LDH法进行研究。结果细胞经黄药子醇提物处理24 h、48 h后,测得黄药子醇提物的IC50为20.284,19.453μg/ml,乳酸脱氢酶(LDH)的释放率较小。结论黄药子醇提物的肝毒性可能不是通过破坏其细胞膜造成的。Objective To study the alcoholic extract from Dioscorea bulbifera L. on the inhibitory effect of liver HL-7702 cell growth and the lactate dehydrogenase（ LDH） releasing rate. Methods MTT assay and LDH assay are conducted to test.Results The half maximal inhibitory concentration（ IC50） of the alcoholic extract from Dioscorea bulbifera L. is 20. 284μg/mL,19. 453μg /mL after the cells are processed for 24 hours,48 hours,the LDH releasing rate is lesser. Conclusion Hepatotoxicity of the Alcoholic Extract from Dioscorea bulbifera L. may not primarily through damaging the cell membranes.

关 键 词：黄药子醇提物 MTT法 LDH法 HL-7702细胞 细胞培养 

分 类 号：R285.5[医药卫生—中药学]