半枫荷抗乙型肝炎病毒的药理活性研究  

A study of inhibition effects on hepatitis B virus of semi-cathayensis in vitro

作　　者：孙静[1] 郑雪凌[1] 崔向珍[1] 孟琳[1] 

机构地区：[1]山东商业职业技术学院,山东济南250103

出　　处：《时珍国医国药》2014年第10期2391-2393,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的探索半枫荷抗乙肝病毒的药理活性。方法以HBV-DNA转染的人肝细胞株HepG2为实验对象,用从半枫荷中提取的不同药物组分对细胞进行处理,考察药物处理4天后病毒细胞的存活率和药物对病毒抗原HBeAg、HBsAg的抑制作用。结果半枫荷化学成分全提取物对2种病毒抗原均有抑制作用,但明显低于齐墩果酸纯化品的抑制效果,2个药物组分处理后的细胞存活率也随药物浓度的增加呈下降趋势。除去齐墩果酸的半枫荷有效成分提取物对病毒抗原无抑制作用,说明半枫荷中抗肝炎病毒的活性物质为齐墩果酸。结论半枫荷对病毒性肝炎的抗原具有抑制活性,其抗肝炎病毒活性成分主要为齐墩果酸。Objective To explore the pharmacological activity for anti-hepatitis B virus of semi-cathayensis. Methods The HBV-DNA transfected human liver cell line HepG2 as experimental subjects,cells were treated with different drug components extracted from the semi-cathayensis,investigated the rate of virus-cell survival and the inhibition effect for viral antigen HBeAg and HBsAg 4 days later. Results The full components which extracted from semi-cathayensis had a inhibition effect for the two kinds of virus antigen,but lower than the effect of the purified product of oleanolic acid,the virus cell survival rate had a downward trend with the increasing of the concentration of drug components. The drug components which removed oleanolic acid had no inhibitor effect for viral antigen,it suggested that the effective ingredients which could anti-hepatitis B virus was oleanolic acid.Conclusion Semi-cathayensis had inhibitory activity for hepatitis antigen,the active ingredient which anti-hepatitis virus was mainly oleanolic acid.

关 键 词：半枫荷 齐墩果酸 肝炎 药理活性 

分 类 号：R285.5[医药卫生—中药学]