田蓟苷在大鼠体内血药浓度的高效液相色谱法测定及其药动学研究  

Determination of tilianin in rat plasma by HPLC and its application to pharmacokinetic study

作　　者：袁勇[1] 陈卫军[1] 邢建国 王立萍[3] 王新春[1,3] 

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832008 [2]新疆维吾尔自治区药物研究所,新疆乌鲁木齐830004 [3]石河子大学药学院,新疆石河子832002

出　　处：《时珍国医国药》2014年第10期2555-2557,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金新疆联合基金重点项目(U1203204);兵团博士基金(No.2013B014)

摘　　要：目的建立大鼠血浆中田蓟苷含量的HPLC测定方法,并应用于田蓟苷在大鼠体内的药代动力学研究。方法色谱柱为AgilentC18（4.6 mm×250mm,5μm）,流动相为乙腈-0.4%磷酸水（34：66）,流速1.0mL·min-1,检测波长327nm,柱温35℃。采用HPLC法测定给药后大鼠血浆中田蓟苷的浓度,采用DAS2.0软件拟合并计算其药代动力学参数。结果大鼠血浆中田蓟苷在0.031∽1.984μg·mL-1范围内线性关系良好（r=0.9990）,日内及日间精密度小于6%,方法回收率大于96%。提取回收率大于80%,符合生物样品分析要求。大鼠灌胃田蓟苷3个剂量（25,50,100mg·kg-1）后,t1/2β分别为（6.324±0.319）,（6.099±1.464）,（9.296±2.218）h;AUC（0-t）分别为（0.658±0.019）,（1.050±0.056）,（1.380±0.043）mg·L-1·h-1;Tmax分别为（0.278±0.127）,（0.306±0.096）,（0.361±0.048）h;Cmax分别为（0.314±0.016）,（0.566±0.031）,（0.747±0.050）mg·L-1。结论该法灵敏度高,专属性强,准确可靠,适用于田蓟苷大鼠血药浓度的测定及其药代动力学研究。Objective To establish an HPLC method for the determination of tilianin in rat plasma and to investigate the pharmacokinetics of tiliani in rats. Methods The analysis was performed on an AgilentC18 column（ 4. 6 × 250 mm,5μm） at 35℃ with the mobile phase of acetonitrile-0. 4% phosphoric acid solution（ 34： 66,v /v） at a flow rate of 1. 0ml·min-1. The detecting wavelength was set at 327 nm. The concentrations of tiliani in plasma were measured by using HPLC method and the pharmacokinetic parameters were fitted and calculated by the software of Drug and Statistics 2. 0. Results The calibration curve of tilianin was linear in the range of 0. 031 ∽ 1. 984μg·mL-1with the correlation coefficient of 0. 9990. The intra-day precision and the inter-day precision were less than 6%. The recoveries were more than 96%. The extraction recoveries were more than 80%. The t1 /2β of tilianin after oral administration with three different doses to rats were（ 6. 324 ± 0. 319）,（ 6. 099 ± 1. 464）,（ 9. 296 ±2. 218） h; AUC（ 0-t） were（ 0. 658 ± 0. 019）,（ 1. 050 ± 0. 056）,（ 1. 380 ± 0. 043） mg·L-1·h-1; Tmax were（ 0.278 ±0.127）,（ 0. 306 ± 0. 096）,（ 0. 361 ± 0. 048） h; Cmax were（ 0. 314 ± 0. 016）,（ 0. 566 ± 0. 031）,（ 0. 747 ± 0. 050） mg·L-1.Conclusion The method is proved to be accurate and suitable for the pharmacokinetic study of tilianin.

关 键 词：田蓟苷 药代动力学 高效液相色谱法 

分 类 号：R285.5[医药卫生—中药学]