UPLC法测定抗肿瘤药物甲氨蝶呤血药浓度  

Determination of Plasma Concentration of Methotrexate by UPLC

作　　者：张建英[1] 代宏剑[1] 周敬静[1] 温柏平[1] 张桢[1] 

机构地区：[1]昆明市儿童医院检验科,云南昆明650034

出　　处：《临床医学工程》2014年第10期1248-1249,共2页Clinical Medicine & Engineering

摘　　要：目的：临床上建立一种快速、准确测定肿瘤患儿血浆中甲氨蝶呤浓度方法。方法取肿瘤患儿血浆250μ蕴，加入7%高氯酸200μ蕴，涡旋1皂蚤灶，室温高速离心5皂蚤灶（15000×早），取上清液5μ蕴进样分析。色谱条件：色谱柱为月耘匀悦18柱（50皂皂×2.1皂皂，1.7μ皂）；流速为0.3皂蕴·皂蚤灶-1；流动相为乙腈-40皂燥造·蕴-1磷酸二氢钾缓冲液（91：9，责匀越4.5）；柱温为30℃；进样量：5μ蕴；检测波长为304灶皂。结果血浆中M栽载色谱峰分离良好，无干扰，曲线为赠越41729曾-810.29，砸2越0.9997，回收率在90%--110%之间。结论哉孕蕴悦操作简便，适用于血浆中M栽载快速监测。Objective To establish a rapid and accurate method to determine the plasma concentration of methotrexate in children with tumor. Methods 0.25μL plasma specimen taken from tumorous children was extracted with 7%perchloric acid and swirled 1 min, then it was high-speed centrifuged 5 min （15 000μg）. 5μL supernate was injected in UPLC assay for analysis. The analytical column was BEH C18 （50 mmμ2.1 mm, 1.7μm）, the flow rate was 0.3 mL·min-1, and the mobile phase was the buffer of acetonitrile-40 mol·L-1 and NaH2PO4 （91： 9, pH = 4.5）. The column temperature was 30 ℃, the sample size was 5 μL, and the wavelength was 304 nm. Results The chromatographic peak of methotrexate in plasma separated good without interference. The curve was y=41 729x-810.29, R2=0.9 997, the recycle rate was between 90%and 100%. Conclusions UPLC is easy to operate and fit for fast determination of MTX in human plasma.

关 键 词：甲氨蝶呤 高效液相色谱法 血浆 

分 类 号：R917[医药卫生—药物分析学]