生物羊膜免疫安全性的筛检  

Screening of biological amniotic immunization safety

作　　者：荣玲[1,2] 赵雄[2] 裴秀娟 晏飞燕 马玉媛[2] 方迟[2] 付守廷[1] 章金刚[2] 

机构地区：[1]沈阳药科大学药理学教研室,沈阳110016 [2]军事医学科学院野战输血研究所,北京100850 [3]天津世纪康泰生物工程有限公司,天津300457

出　　处：《国际药学研究杂志》2014年第5期575-579,共5页Journal of International Pharmaceutical Research

摘　　要：目的探讨生物羊膜对大鼠免疫安全性的影响。方法将150只Wistar大鼠随机分为生物羊膜、免疫抑制、免疫刺激、假手术和空白对照5组,依据考察周期将每组30只再随机分为1、2、4、8和12周5个实验操作小组。各组大鼠皮下埋植后连续3 d肌内注射硫酸庆大霉素抗感染,按考察周期进行免疫器官系数分析、NK细胞杀伤活性测定及IL-1β、IL-6和TNF-α含量测定。结果生物羊膜植入后1、2、4、8和12周,生物羊膜与假手术和空白对照组大鼠各项检测结果相比不存在明显差异(P>0.05);术后1周,免疫抑制组大鼠免疫器官系数分析等出现不同程度的免疫抑制,与其余各组相比存在显著性差异(P<0.01),免疫刺激组大鼠NK细胞杀伤活性等出现一定的免疫刺激,与其余各组相比存在明显差异(P<0.05)。结论生物羊膜植入大鼠体内后具有较好的免疫安全性,未产生明显的不良免疫反应。Objective To evaluate the immunity efficacy of human amniotic membranes on rats. Methods One hundred and fifty Wistar rats were randomly divided into five groups：biological amnion group,immunosuppression group,immunostimulation group,sham-operated group and blank control group. According to the study period,each group of thirty rats would be randomly divided into five experimental operation subgroups：the 1st week,the 2nd week,the 4th week,the 8th week group and the 12 th week groups. The rats were implanted subcutaneously,then intramuscular injection of gentamicin sulfate for 3 days to resist the infection,and the immune organ coefficient,and the killing abilities of NK cell,IL-1β,IL-6 and TNF-αserum levels were detected according to the study period.Results At 1st,2nd,4th,8th and 12 th week after amniotic membrane implantation in rats,compared with the sham-operated and blank control groups,the biological amnion group had nonsignificant differences（P0.05）. At 1st week after amniotic membrane implantation in rats,immunosuppression group showed different levels of the immunosuppressive effect,such as the analysis of immune organ coefficient,which had significant differences compared with other groups（P0.01）. At 1st week after amniotic membrane implantation in rats,the imunostimulation group showed a certain degree of the immunostimulant effect,such as the killing abilities of NK cell,which had marked differences compared with other groups（P0.05）. Conclusion The amniotic membranes have satisfactory immune safety with implantation in rats and do not cause significant adverse immune reactions.

关 键 词：生物羊膜 免疫刺激 免疫抑制 免疫安全性 免疫毒性 

分 类 号：R318.08[医药卫生—生物医学工程]