辽宁省2012年至2013年呼吸道腺病毒型别鉴定  

Genotyping of respiratory adenovirus between 2012 and 2013 in Liaoning

作　　者：张眉眉[1] 于伟[1] 孙海波[1] 姚文清[1] 

机构地区：[1]辽宁省疾病预防控制中心感染与传染病所,辽宁沈阳110005

出　　处：《中国微生态学杂志》2014年第8期893-895,共3页Chinese Journal of Microecology

基　　金：国家重大传染病防治科技重大专项(2012ZX10004209-001)

摘　　要：目的基于国家重大传染病防治科技重大专项项目平台,对2012年1月至2013年12月间辽宁省采集的发热伴呼吸道症状患者标本进行检测,了解腺病毒的流行情况并对其型别进行分析。方法采集具有发热伴呼吸道症状患者的咽拭标本470份,经多重PCR检测,检出阳性标本30份,选取阳性标本进行病毒分离,共获得人类腺病毒(Human Adenovirus,HAdv)毒株20株。将所得腺病毒的核酸进行巢式PCR扩增并进行测序,获得Hexon基因序列。将基因序列在NCBI进行Blast比对。结果在分离的得到的20株腺病毒毒株中包含HAdv-2型6株,HAdv-3型11株,HAdv-5型2株,HAdv-14型1株。结论2012至2013年间,辽宁省引发发热伴呼吸道症状的腺病毒型别以2型和3型为主,同时也有少量其他型别检出。Objective To learn the prevelance of Adenovirus and analyze its genotypes. Methods 470 samples were collected from patients with fever and respiratory symptoms in Liaoning during Jan 2012 and Dec 2013,among which 30 samples were positive in Adenovirus test. 20 strains of human adenovirus were isolated from those positive samples; Sequencing and blasting was performed after PCR. Results There were 6 strains of Adv-2 type,11 strains of Adv-3 type,2 strains of Adv-5 type and 1 strain of Adv-14 type. Conclusion The main genotypes of Adenovirus was Adv-2 and Adv-3 in Liaoning between 2012 and 2013.

关 键 词：PCR反应 人类腺病毒 Hexon基因 

分 类 号：R511.8[医药卫生—内科学]