基于酵母报道基因系统检测珠江三角洲水域二噁英污染物的分布蓄积特征  

Investigating distribution and accumulation of dioxin-like pollutants with yeast genetic reporter system in rivers of the Pearl River Delta

作　　者：黄楚琴[1] 蔡文泳[2] 刘寒英[3] 李冰[1] 王维华[4] 黄莉[3] 

机构地区：[1]广州医科大学实验医学研究中心,广州510182 [2]广州医科大学公共卫生学院,广州510182 [3]广州医科大学实验动物研究中心,广州510182 [4]广州医科大学第三附属医院,广州510150

出　　处：《应用与环境生物学报》2014年第4期690-696,共7页Chinese Journal of Applied and Environmental Biology

基　　金：广东省自然科学基金项目(S2011010005438);国家自然科学基金项目(81270745)资助~~

摘　　要：利用酵母报道基因系统,检测广州市内河涌水体、珠江三角洲周围典型淡水、珠江口水体以及对应底泥样品中的二噁英类似物,探讨二噁英污染类似物在珠江三角洲水域的分布蓄积和来源特征.结果表明,广州市区流溪河同德围涌段、荔河芳村码头涌段水体存在较严重的污染,2,3,7,8-四氯代二苯并-对-二噁英(2,3,7,8-tetrachlorodibenzopdioxin,TCDD)毒性当量分别达(50.189 0±5.695 4)ng/L、(13.096 9±0.825 2)ng/L;广州市区河涌底泥污染分布水平表现为荔河涌段>番禺涌段>天河涌段;珠江三角洲周围水体及对应底泥污染蓄积主要分布在东江惠州段和西江肇庆段,而北江段和潭江段各采样点未见明显污染.研究表明珠江三角洲水域二噁英污染物的蓄积呈非均匀分布,城市和城市周边工业区河涌及其流经珠江河段污染较重,说明污染主要来源于小型加工和化工工业活动.This research aimed to explore the source, distribution and accumulation characteristics of dioxin-like pollutants in the Pearl River Delta. Dioxin-like substances content in water and corresponding sediment samples from canals in Guangzhou City and River estuary in the Pearl River Delta were detected by utilizing a recombinant yeast gene reporter system. The Tongdewei and Fangcun Dock canal waters in Guangzhou City were severely polluted with dioxins, with toxicity equivalency quantity as 50.189 0 ± 5.695 4 ng/L and 13.096 9 ± 0.825 2 ng/L respectively. The sediment samples collected from Lihe, Panyu and Tianhe canals contained the highest dioxin-like pollutants in the order of Lihe〉 Panyu 〉Tianhe. In the Huizhou reach of East River and the Zhaoqing reach of the West River, dioxin pollutants were detected with high concentration in both water and sediment samples, while no distinct pollutants were detected in samples collected from sites of the North River or the Tanjiang River. This study showed that the dioxin pollutants are differentially distributed in the Pearl River Delta, with more accumulation in city canals and river reaches around cities. These results demonstrated that the source of dioxin pollutants may be the small-scale fabrication and chemical industries.

关 键 词：二噁英类似物 珠江三角洲 水体污染 底泥污染蓄积 酵母报道基因系统 

分 类 号：X832[环境科学与工程—环境工程]