Zalerion varium接种对蓝莓扦插苗生根及生长的影响  

Effect of Zalerion varium Inoculation on the Rooting and Growth of Blueberry Cutting Seedling

作　　者：刘凤红[1] 程显好[2] 顾亮[3] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025 [2]鲁东大学农学院,山东省食用菌技术重点实验室,山东烟台264025 [3]烟台市农业科学研究院,山东烟台264025

出　　处：《北方园艺》2014年第20期100-104,共5页Northern Horticulture

基　　金：山东省科技发展计划资助项目(ZR2012GSF12110);山东省科技发展计划资助项目(2012YD11009)

摘　　要：在温室营养穴盘栽培条件下,以蓝莓"杜克"品种的无根扦插苗和真菌Zalerion varium液体培养物为试材,采用3种接种方法(基部接种、浸泡接种和喷雾接种),研究了不同接种方法处理下接种真菌Zalerion varium和未接种之间"杜克"蓝莓扦插苗的根系菌根侵染率、地上部分鲜重、根系总长、根表面积、叶绿素荧光参数等特征的差异。结果表明:采用3种接种方法,Zalerion varium均可不同程度地定殖与侵染蓝莓扦插苗的根系;接种Zalerion varium真菌显著促进宿主蓝莓扦插苗的生根和生长,与未接种对照相比,接种"杜克"蓝莓扦插苗的地上部分鲜重、根系总长和根系表面积以及叶绿素荧光参数Fv/Fo和Fv/Fm均显著提高;接种Zalerion varium真菌后,可显著促进蓝莓扦插苗的生根和生长,但3种接种方法有显著差异,其中基部接种和浸泡接种2种方法比喷雾接种方法更为有效。Taking ＇Duke＇ blueberry cutting seedling and Zalerion varium as materials, three inoculation methods were applied in greenhouse：applying liquid cultures to the suhstrate, spraying liquid Zalerion varium cultures into the substrate and soaking the unrooted tissue culture plantlets for 20 minutes. The root colonization, the shoot biomass, the root length and the root surface area and the chlorophyll fluorescence parameters Fv/Fo and Fv/Frn were compared between plants infected with Zalerion variurn and those not infected under different inoculation treatments. The results showed that plants became naturally colonized by Zalerion varium with three inoculation methods, however, the intensity of root colonization were different. The rooting and growth of cutting seedlings were remarkably promoted. Compared to the non- inculation plants,the shoot biomass,the root length and the root surface area and the chlorophyll fluorescence parameters Fv/Fo and Fv/Fm were increased sinificantly. Compared to spraying liquid Zalerion variurn cultures into substrate, applying liquid cultures to the culture substrate and soaking the cuttings for 20 minutes were more effective.

关 键 词：Zalerion varium 接种方法 “杜克”蓝莓扦插苗 生根 生长 

分 类 号：S663.915[农业科学—果树学]