辽宁省西瓜炭疽病病原菌鉴定及生物学特性研究  

Identification and biological characteristics of watermelon anthracnose caused by Colletotrichum orbiculare in Liaoning Province

作　　者：唐爽爽[1] 刘志恒[1] 余朝阁[1] 郑川[1] 安心[1] 李健冰 赵廷昌[2] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866 [2]中国农业科学院植物保护研究所,北京100193

出　　处：《植物保护》2014年第4期38-44,共7页Plant Protection

基　　金：国家西甜瓜产业技术体系(CARS-26)

摘　　要：针对西瓜炭疽病病原菌进行了鉴定及其生物学特性研究。通过病原菌形态学鉴定,柯赫氏法则证病,rDNAITS测序,鉴定该病致病菌为瓜类炭疽菌(Colletotrichum orbiculare)。生物学特性测定结果表明:病菌菌丝生长最适培养基为PSA;最适碳、氮源分别为蔗糖和NH4Cl;最适温度为25℃;最适pH为6;黑暗条件利于病菌生长。病菌产孢最适培养基为西瓜茎叶煎汁培养基;最适碳、氮源分别为蔗糖和(NH4)2SO4;最适温度为25℃;最适pH为6;黑暗条件利于孢子产生。菌丝致死温度为55℃,10min;分生孢子致死温度为52℃,10min。The watermelon anthracnose pathogens and their biological characteristics were studied.By morphological identification,card of Koch＇s postulation,rDNA-ITS sequencing,the watermelon anthracnose pathogen Colletotrichum orbiculare was identified.Biological characteristics measurement showed that PSA was the optimal medium for hyphal growth.The optimum carbon and nitrogen sources were sucrose and NH4Cl;the optimal temperature was 25℃;the optimal pH value was 6.The dark condition was conducive to bacterial growth.Fried juice of watermelon stems and leaves was the optimal culture medium for bacterial spores,and the optimum carbon,and nitrogen sources were sucrose and（NH4）2SO4;the optimal temperature was 25℃ and the optimal pH value was6;dark condition was conducive to spores.Mycelial lethal temperature was 55℃for 10 min,and conidial lethal temperature was 52℃for 10 min.

关 键 词：西瓜炭疽病 瓜类炭疽菌 鉴定 rDNA-ITS测序 生物学特性 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]