河南省番茄灰霉病菌对3种杀菌剂的抗药性检测  

Detection of the resistance of Botrytiscinereaon tomato in Henan Province to three fungicides

作　　者：刘圣明[1] 高续恒 张艳慧[1] 李梦[1] 梅笑寒 

机构地区：[1]河南科技大学林学院植物保护系,洛阳471003

出　　处：《植物保护》2014年第4期144-147,共4页Plant Protection

基　　金：国家自然科学基金青年科学基金(31301688);公益性行业(农业)科研专项(201303023);河南省教育厅科学技术研究重点项目(13A210277);河南科技大学博士科研启动基金(09001589)

摘　　要：为了明确河南省番茄灰霉病菌对苯并咪唑类杀菌剂多菌灵、二甲酰亚胺类杀菌剂腐霉利和氨基甲酸酯类杀菌剂乙霉威的抗药性状况,2013年从河南省不同保护地中采集番茄灰霉病病果或病叶,经单孢分离共获得番茄灰霉病菌菌株139株。采用最低抑制浓度法(MIC)测定了其对多菌灵、腐霉利和乙霉威的抗药性。结果显示:番茄灰霉病菌对多菌灵、腐霉利和乙霉威产生抗性菌株的频率分别为81.29%、80.58%和93.53%;所测菌株对3类杀菌剂的抗性类型有BenRDicSNPCS、BenRDicRNPCS、BenRDicSNPCR、BenSDicRNPCR、BenSDicSNPCR和BenRDicRNPCR6种,所占比例分别为2.88%、3.60%、3.60%、5.04%、13.67%和71.22%。表明河南省番茄灰霉病菌已对多菌灵、腐霉利和乙霉威产生抗药性,迫切需要筛选新的杀菌剂防治番茄灰霉病。To investigate the resistance of Botrytis cinerea to carbendazim,procymidone and diethofencarb,one hundred and thirty-nine strains of B.cinerea were collected from the diseased tomato fruits or leaves in different regions of Henan Province in2013,and resistance to the three fungicides were detected by minimal inhibitory concentration（MIC）method.The results showed that the percentages of carbendazim resistance,procymidone resistance and diethofencarb resistance were 81.29%,80.58% and 93.53%,respectively.The resistance types were Ben^RDic^SNPC^S、Ben^RDic^RNPC^S、Ben^RDic^SNPC^R、Ben^SDic^RNPC^R、Ben^SDic^SNPC^R and Ben^RDic^RNPC^R,accounting for 2.88%,3.60%,3.60%,5.04%,13.67%and 71.22%,respectively.These results suggest that B.cinerea on tomato plants in Henan has produced resistance to carbendazim,procymidone and diethofencarb,therefore,it＇s urgent to apply new fungicide to control B.cinerea.

关 键 词：灰霉病 灰葡萄孢菌 杀菌剂 抗药性 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]