慢性肾脏病患者血浆S-腺苷同型半胱氨酸水平与基因组DNA甲基化的关系  

The correlaion between plasma S-adenosylhomocysteine and global DNA methylation in patients with chronic kidney disease

作　　者：欧三桃[1] 刘琦[1] 陈昕[1] 李莹[1] 刘建[1] 

机构地区：[1]泸州医学院附属医院肾病内科,四川泸州646000

出　　处：《广东医学》2014年第13期2031-2033,共3页Guangdong Medical Journal

基　　金：四川省泸州市科技局资助项目(编号:泸市科函[2011]42号)

摘　　要：目的研究慢性肾脏病(CKD)患者血浆S-腺苷同型半胱氨酸(SAH)水平与整体基因组DNA甲基化的关系。方法选择CKD患者43例和健康对照组36例,ELISA法检测血清Hcy浓度,酶连续循环比色法检测血清SAH浓度,高效液相色谱检测基因组DNA甲基化程度。结果 CKD患者血浆SAH较健康对照组明显升高,而基因组DNA甲基化水平降低。血浆SAH浓度与基因组DNA甲基化呈明显负相关。结论 CKD患者血浆SAH水平升高可导致异常的基因组DNA甲基化,可能参与CKD患者心血管并发症的发生发展。Objective To assess the correlaion between plasma S- adenosylhomocysteine（SAH） and DNA methylation in patients with chronic kidney disease（ CKD）. Methods Forty- three patients with CKD and 36 healthy volunteers were included in this study. Plasma Hcy and SAH levels were measured by ELISA and continuous cycle enzyme colorimetric method,respectively. DNA methylation was assessed using HPLC. Results The plasma SAH in CKD patients was significantly higher than that in the healthy controls while DNA methylation decreased. Plasma SAH was negatively correlated with global DNA methylation in patients with CKD. Conclusion High plasma SAH concentration may relate to impaired DNA methylation and mediates the development of cardiovascular complications in patients with CKD.

关 键 词：慢性肾脏病 S-腺苷同型半胱氨酸 DNA甲基化 

分 类 号：R593.22[医药卫生—内科学]