miRNA-208b在SD大鼠腹主动脉缩窄心肌肥厚中的表达  

Dynamic different expression of miRNA- 208b in cardiac hypertrophy model induced by abdominal aortic coarctation in SD rats

作　　者：吴菊清[1] 欧阳伟[1] 冯会娟[1] 孙云钢[1] 李师思[1] 陈盼[1] 冼嘉朗[1] 黄柳华[1] 

机构地区：[1]南方医科大学珠江医院核医学科,广州510282

出　　处：《广东医学》2014年第14期2141-2144,共4页Guangdong Medical Journal

基　　金：广东省自然科学基金资助项目(编号:10151051501000036);广东省科技计划项目(编号:2012B031800125)

摘　　要：目的建立腹主动脉缩窄(AAC)大鼠心肌肥厚动物模型并分析大鼠心肌肥厚过程中microRNA-208b(miRNA-208b)动态差异性表达。方法大鼠40只,平均分为AAC组和假手术组(Sham组),AAC组通过AAC的方法建立大鼠心肌肥厚模型,采用miRNA芯片法筛查和实时荧光定量PCR技术(qRT-PCR)分别检测AAC组与Sham组大鼠心肌中miRNA-208b表达的动态变化。结果 (1)心肌肥厚模型的建立:1心脏指数变化:AAC组大鼠在5、10、15、20 d心脏指数均值稳步上升,与Sham组相比,除5 d时心脏指数差异无统计学意义(P>0.05),10、15、20 d心脏指数差异有统计学意义(P<0.05)。2组织学染色示:AAC组5 d时大鼠心肌细胞排列正常,无明显肥大现象;10 d时大鼠部分心肌细胞排列紊乱,体积增大;15、20 d时大鼠心肌组织排列紊乱,心肌细胞明显肥大,胞浆丰富,细胞间隙不明显,细胞核增大。此均可证明大鼠心肌肥厚模型建立成功。(2)miRNA-208b基因芯片筛查及qRT-PCR检测:1基因芯片筛查实验结果显示,AAC组大鼠心肌中miRNA-208b在5、10、15、20 d分别是Sham组的0.828 2、1.951 4、1.291 9、1.329 6倍,高峰在10 d出现。2qRT-PCR结果显示,AAC组大鼠心肌中miRNA-208b在5、10、15、20 d分别为Sham组(设Sham组中的miRNA-208b表达量为1)的相对表达倍率是(2.65±0.49)、(1.27±0.31)、(1.34±0.69)、(3.45±0.27)倍。与Sham组相比,AAC组miRNA-208b表达在5 d和20 d时差异均有统计学意义(P<0.05);但在10 d和15 d时差异均无统计学意义(P>0.05)。结论在AAC大鼠中,miRNA-208b表达呈动态差异性变化,且在早期表达较高,先于心肌细胞发生形态学变化,可能成为心肌肥厚早期诊断和干预治疗的新途径。Objective To establish myocardial hypertrophy animal model induced by partial abdominal aorta coarctation（AAC） and to investigate the dynamic expression of microRNA- 208b（miRNA- 208b） in SD rats. Methods Forty SD rats were randomly divided into the AAC group,in which myocardial hypertrophy was induced by AAC,and sham- operation group（Sham group）. The dynamic expression of miRNA- 208 b was measured by microarray assay and real- time quantitative reverse transcription- PCR（qRT- PCR） on the 5^th,10^ th,15thand 20 ^thday. Results The myocardial hypertrophy model was successfully established. The cardiac indexes in AAC group rose steadily on the 5^th,10^ th,15^thand 20^ thday,significantly higher than those in Sham group except for on the 5thday（P〈0. 05）. Although normal arrangement and shape of myocardial cells were observed in AAC group on the 5thday; disordered arrangement and mild hypertrophy and blurred intercellular space was observed on the 15 ^thand 20^thday. According to microarray assay,the miRNA- 208 b in AAC group were 0. 828 2,1. 951 4,1. 291 9 and 1. 329 6 times of those in Sham group on the 5th,10 th,15thand 20 thday,respectively. According to qRT- PCR,the relative expression ratios of miRNA- 208 b in AAC group to Sham group were（2. 65 ± 0. 49）,（1. 27 ± 0. 31）,（1. 34 ± 0. 69） and（3. 45 ± 0. 27） on the 5^th,10^ th,15^thand 20 th day,respectively. Significant differences in miRNA- 208 b expression were revealed between AAC and Sham groups on the 5^thand 20^thday（P〈0. 05）. Conclusion In AAC rats,the miRNA- 208 b is dynamically changed and up- regulated in early stage myocardial hypertrophy,prior to morphological changes in myocardial cells,suggesting a novel early diagnosis and intervention target for myocardial hypertrophy.

关 键 词：微小RNA 心肌肥厚 PCR 基因芯片 早期诊断 动物模型 

分 类 号：R542.2[医药卫生—心血管疾病]