萘降解菌TN培养基组成及条件优化  

Optimization of the medium components and the culture condition of naphthalene-degrading strain TN

作　　者：马欣[1] 刘蕊[1] 柴铭峰 袁婧楠 刘金彪[1] 廉景燕[1] 黄磊[1] 

机构地区：[1]天津理工大学化学与化工学院,天津300384

出　　处：《天津理工大学学报》2015年第1期56-60,共5页Journal of Tianjin University of Technology

基　　金：国家自然科学基金青年基金(31100400)

摘　　要：从大庆油田石油污染的土壤中筛选得到一株萘降解菌株TN,经初步鉴定为伯克霍尔德氏菌属(Burkholderiasp.),采用单因素试验设计优化了菌株TN降解萘的培养基组成及培养条件.结果表明,菌株TN的最适培养条件为:碳源(萘)浓度为0.5%、氮源选择(NH4)2SO4浓度为2.64g/L、磷源为KH2PO4∶Na2HPO4摩尔比为3∶1、酵母粉浓度为0.03g/L、MgSO4浓度为0.7g/L、培养时间为3d、培养温度为30℃、pH=9、装液量为30mL、接种量为2%、摇床转速为200rpm.验证实验和预期一致,优化前萘的降解率为62.03%,优化后萘的降解率可达到97.95%,整体提高了35.92%.An efficient naphthalene-degrading strain TN was isolated from oil contaminated soil of Daqing Oil Field and identified as Burkholderia sp., which could use naphthalene as sole carbon source. The single factor experiments were used to optimize the medium components and the culture condition. It was found that the optimal culture condition for strain TN is carbon source（naphthalene） 0.5%, nitrogen source（NH4）2SO42.64g/L, phosphorus source KH2PO4∶ Na2HPO4= 3∶1, yeast extract0.03g/L, Mg SO40.7g/L, optimal culturing time 3 days, optimal temperature 30 ℃, initial pH 9, liquid volume 30m L, inoculation amount 2% and shaking speed 200rpm. The actual experimental results were in agreement with the prediction. The biodegradation rate of naphthalene increased from 62.03% to 97.95% after optimization.

关 键 词：生物修复 伯克霍尔德氏菌 单因素 萘降解 

分 类 号：X53[环境科学与工程—环境工程]