拉曼散射光谱快速鉴别替硝唑葡萄糖注射液  

Rapid identification of tinidazole and glucose injection by Raman scattering spectra

作　　者：王春红 

机构地区：[1]湖北省食品药品监督检验研究院,湖北武汉430064

出　　处：《中国卫生检验杂志》2014年第13期1866-1867,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的本文采用拉曼光谱分析技术，对替硝唑葡萄糖注射液进行拉曼光谱扫描，采用模式识别技术，建立样品的快速鉴别模型。方法采用拉曼散射光谱法，激发波长785nm，激光强度和积分时间根据样品的特性进行调整，对收集的替硝唑葡萄糖注射液样品进行拉曼光谱扫描，以替硝唑、葡萄糖为两个主成分，选择特征谱段，建立化学计量学模型：谱段为970cm-1～1140cm-1与1160cm-1～1580cm-1；光谱预处理方法为矢量归一与一阶导数（30点平滑）；采用主成分光谱和主成分参考光谱的相关系数进行计算；阈值为0．95，同时验证模型的准确性。结果所建立的鉴别模型对验证集中的样品能够作出准确无误的判别。结论通过验证试验表明，模型结果准确可靠，所建方法准确有效，可用于替硝唑葡萄糖注射液的快速鉴别，适用于市场监督抽样。Objective To establish a rapid identification model of tinidazole and glucose injection by Raman scattering spectra based on scanning and mode recognition. Methods The excitation wavelength of Raman scattering spectrum method was set at 785 nm, and the laser strength and integral time were adjusted according to the characteristic of the samples. The tinidazole and glucose injection were scanned by Raman spectrum, the characteristic frequency regions were selected by using tinidazole and glucose as main components to establish the chemical metrology model. The frequency regions were 970 cm-1 ~ 1 140 cm-1 and 1 160 cm -1 - 1 580 cm -1 , the pretreatment methods of spectrum were First Derivative（ smoothing points 30） and Vector Nor- malization. The correlation coefficient of the spectra and reference spectra of principal components was calculated. The threshold was 0.95 and the accuracy of model was verified. Results The results showed that the estabhshed model can distinguish all samples accurately. Conclusion The validation test showed the model restdts were reliable and the method was convenient, fast and effective, so it can be used for quick discrimination of tinidazole and glucose injection and market sampling inspection.

关 键 词：拉曼散射光谱 替硝唑葡萄糖注射液 鉴别 

分 类 号：O657.37[理学—分析化学]