2009年深圳市H3亚型流感病毒分子变异研究  

Study on molecular variation of influenza H3 virus (2009) in Shenzhen

作　　者：黄福新[1] 吕星[1] 程小雯[1] 吴春利[1] 

机构地区：[1]深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国卫生检验杂志》2014年第13期1922-1924,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的了解2009年深圳市H3亚型流感病毒系统进化及分子变异情况。方法用狗肾传代细胞(MDCK)进行流感病毒分离,收获病毒后提取病毒RNA,进行逆转录-聚合酶链式反应(RT-PCR),扩增产物经琼脂糖凝胶电泳鉴定后送大连宝生物公司纯化及测序,测序结果用Clustal W2软件和MEGA 3.1软件进行序列分析。结果 2009年的深圳毒株与同期国际疫苗株"A/Brisbane/10/2007"相比差异较大,在HA1区段共发生了7个氨基酸位点的变异:62位的E突变成了K;抗原决定簇A的144位点由N变成了K;抗原决定簇B的158位点由K变成了N;173位的K变成了Q;抗原决定簇B的189位点由N变成了K;194位的P变成了L;213位的V突变成了A。值得注意的是N144K突变导致了144位糖基化位点的消失,这些突变可能已经造成了病毒抗原性的改变。结论 2009年深圳市流行的H3亚型流感病毒很可能是一个新的优势流行株。Objective To study the phyletic evolution and molecular variation of 2009 influenza H3 virus in Shenzhen.Methods Influenza H3 virus was isolated by MDCK cell culture.Then HA1 genes were amplified by RT-PCR.After sequencing of amplification products,phyletic evolution and molecular variation of HA1 gene were analyzed by ClustalW2 and MEGA3.1 softwares.Results Significant variation of 2009 influenza H3 viruses in Shenzhen were observed when compared to the 2009 vaccine strains＂A/Brisbane/10/2007＂.A total of 7 amino acid mutations occurred on the HA1 segment：E62K,N144K,K158N,K173Q,N189K,P194L,V213A.Among them,three mutations（N144K,K158N,N189K） were located on the antigenic determinants A and B;a glycosylation site disappeared due to the N144K mutation.These mutations may have induced the antigenic changes of 2009 influenza viruses.Conclusion The 2009 influenza H3 virus prevalent in Shenzhen was likely to be a new predominant epidemic strain.

关 键 词：H3亚型 流感病毒 分子变异 

分 类 号：R373.13[医药卫生—病原生物学]