基于自组装原位生长法制备聚苯胺/CNTs纳米复合物修饰叉指电极的葡萄糖生物传感器  

Glucose biosensor based on self assembled-in situ polymerized polyaniline /CNTs nanocomposite modified interdigitalelectrode

作　　者：孙建东 马振华[2] 姚晓霞[2] 赵修青 李海涛[2] 王建伟[2] 柴秀琴[3] 王会才[1,2,3] 

机构地区：[1]天津兰力科化学电子高技术有限公司,天津300143 [2]天津工业大学环境与化学工程学院,天津300387 [3]天津工业大学中空纤维膜材料与膜过程国家重点实验室培育基地,天津300387

出　　处：《天津工业大学学报》2015年第1期17-21,共5页Journal of Tiangong University

基　　金：国家自然科学基金(30900325);天津市以及科技部中小企业创新基金(13ZXCXSY14200,13C26211200305)

摘　　要：采用自组装原位生长制备基于聚苯胺/CNTs 纳米复合物修饰叉指电极的葡萄糖生物传感器. 首先采用自组装的方式制备CNTs 修饰叉指电极,并采用对甲苯磺酸掺杂聚苯胺,原位在修饰电极表面生长聚苯胺,制备了PANI/CNTs 修饰电极; 然后将葡萄糖氧化酶固定在修饰电极表面制备了葡萄糖生物传感器. 采用拉曼光谱、扫描电子显微镜、原子力显微镜等对所制备的PANI/CNTs 复合物进行了表征分析;采用循环伏安法和电化学阻抗法研究了修饰电极的电化学行为. 传感器性能评价结果表明：在最优化条件下,葡萄糖浓度与响应电流在0.5-30 mmol/L 范围内呈现良好的线性关系, 响应灵敏度为62.17 μA/（mmol·L^-1）, 线性相关系数为0.997,检出限为0.15 mmol/L（S/N = 3）,并具有良好的重现性和稳定性.Glucose biosensors based on self assembled -in situ polymerized polyaniline /CNTs nanocomposite modifiedinterdigitalelectrode were prepared. Firstly, CNTs modified interdigital electrode was prepared by self assemblingprocess, and PANI/CNTs was prepared using p -toluene sulfonic acid doped polyaniline in situ polymerizedaniline on the electrode surface; then glucose biosensor was prepared by immobilization of glucose oxidase on theas prepared modified electrode surface. The PANI/CNTs nanocomposites were characterized by Raman spectraanalysis, scanning electron microscopy and atomic force microscopy; electrochemical behaviors of the modifiedelectrode were characterized by cyclic voltammetry and electrochemical impedance method. The results showedthat under the optimum conditions, glucose concentration and the response current showed a good linearrelationship in the range of 0.5-30 mmol/L with the response sensitivity of 62.17 μA/（mmol·L^-1）, the linearcorrelation coefficient of 0.997, and the detection limit of 0.15 mmol/L （S/N=3）. The biosensor also showed goodstability and reproducibility.

关 键 词：聚苯胺 碳纳米管 葡萄糖氧化酶 生物传感器 自组装 

分 类 号：TB332[一般工业技术—材料科学与工程] O657.1[理学—分析化学]