力竭运动大鼠苍白球内侧部神经递质浓度及相应受体表达变化研究  

Effect of Exhaustive Exercise on Neurotransmitters and Their Receptors in Rats' Globus Pallidus

作　　者：胡琰茹[1,2] 刘晓莉[1] 乔德才[1] 

机构地区：[1]北京师范大学体育与运动学院,北京100875 [2]中南大学体育教研部,湖南长沙410083

出　　处：《北京体育大学学报》2015年第1期57-62,79,共7页Journal of Beijing Sport University

基　　金：国家自然科学基金资助项目(项目编号:31171138)

摘　　要：目的:通过观察一次性力竭运动过程中大鼠苍白球内侧部(GPi)神经递质谷氨酸(Glu)和γ-氨基丁酸(GABA)及相关受体表达水平的动态变化,探讨力竭运动过程中苍白球内侧部神经元电活动变化的可能机制。方法:选取雄性Wistar大鼠为实验对象,采用微透析-高效液相色谱联用技术,观察一次性力竭运动过程中大鼠苍白球内侧部神经元胞外Glu和GABA水平动态变化;采用免疫荧光双标技术,检测安静、力竭时和恢复90 min时谷氨酸离子型2B受体(NR2B)和γ-氨基丁酸A型受体α1亚基(GABAAα-1)表达水平。结果:自主运动期大鼠苍白球内侧部神经元胞外Glu/GABA比值均显著下降(P<0.05),而力竭即刻其胞外Glu/GABA比值则显著升高(P<0.01)。与安静状态相比,苍白球内侧部在恢复90 min时NR2B受体阳性细胞数量显著增加(P<0.05),力竭即刻和恢复90 min时GABAAα-1受体阳性细胞数量显著减少(P<0.05,P<0.01)。结论:力竭运动过程中大鼠苍白球内侧部Glu、GABA浓度及NR2B、GABAAα-1受体蛋白表达的改变是导致该核团神经元兴奋性变化的原因之一。Objective：The purpose of this study was to reveal the possible mechanism of the neurons activity change in globus pallidus（GPi） during exhaustive exercise by observing the dynamic changes of glutamate（Glu）,γ-aminobutyric acid（GABA） and their receptors in internal segment in rats ＇ GPi during exhaustive exercise.Methods：Male Wistar rats were selected in this study.The expressions of the Glu and GABA in GPi during an exhaustive exercise were observed by microdialysis-high performance liquid chromatogram techniques.The expressions of NR2 B receptor and GABAAα-1 receptor were detected at rest,during exercise,90 min after exercise,by double-lable immunofluorescence technique.Results：Compared with rest state,the Glu / GABA ratio of GPi significantly decreased during the auto-exercise period（P 0.05）,while it was significantly increased at exhaustion time point（P 0.01）.The number of NR2 B positive cells in GPi was increased significantly at 90 min after exhaustive exercise compared with rest state（P 0.05）,but number of GABAAα-1 positive cells was decreased significantly both at exhaustion time point（P 0.05） and 90 min after exhaustion（P 0.01）.Conclusion：The change of expressions of Glu,GABA,NR2 B receptor and GABAAα-1 receptor in rats＇ GPi during exhaustive exercise is a reason for neurons activity change.

关 键 词：力竭运动 大鼠 苍白球内侧部 神经递质 受体表达 

分 类 号：G804.3[文化科学—运动人体科学]