β-catenin在SD大鼠骨关节炎滑膜组织中的表达及意义  

Expression and significance of β-catenin in the synovial of SD rat model of osteoarthritis

作　　者：李建柱[1,2,3,4] 李宽新[3,4] 郭二鹏[1,2,3,4] 史东[1,2,3,4] 孙开军[1,2,3,4] 王维山[1,2] 史晨辉[1,2,3,4] 

机构地区：[1]石河子大学临床医学院,新疆石河子832002 [2]石河子大学医学院地方病实验室,新疆石河子832002 [3]石河子大学医学院第二附属医院 [4]新疆生产建设兵团医院关节脊柱科,新疆乌鲁木齐830000

出　　处：《南京医科大学学报（自然科学版）》2015年第2期155-159,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81160225,81260453,81360451);新疆兵团医药卫生专项资助(2013BA020);兵团国际交流与合作专项资助(2012BC002,2011BC004);兵团科技创新团队专项基金(2014CC002)

摘　　要：目的 :检测并探讨β连环蛋白(β-catenin)在不同时期SD大鼠骨关节炎(osteoarthritis,OA)滑膜组织中的表达及意义。方法:实验组及对照组各随机选取45只SD大鼠,实验组行前交叉韧带切断(ACLT)造模,对照组行假手术处理,两组分别于术后4、8、12周各处死15只,并获取滑膜组织,分别为4、8、12周实验组及对照组;通过免疫组化及半定量RT-PCR技术检测不同时期实验组和对照组滑膜组织中β-catenin蛋白及基因表达水平。结果:免疫组化:β-catenin蛋白在实验组均有表达,且实验组4、8、12周两两相比,β-catenin蛋白表达随周龄增大而依次增高,均具有显著性差异(P<0.05);对照组4周、8周滑膜组织中β-catenin蛋白无明显表达,而部分12周滑膜组织中β-catenin蛋白呈阳性表达。半定量RT-PCR:β-catenin从基因水平在实验组滑膜组织中的表达明显高于对照组,且随着时间的延长,其表达量依次增高,4、8、12周两两比较均有显著性差异(P<0.05)。结论:β-catenin在OA滑膜组织中高表达,且表达量随着时间的延长呈现增高趋势,说明β-catenin与OA的发生及病情进展有关。Objective：To investigate the expression and significance of β-catenin in different times of synovial tissue of osteoarthritis SD rat models. Methods： Ninty SD rats were divided into experimental and control groups randomly. The anterior cruciate ligament （ACL） was cut off to establish a experimental model,the treatment of control group was similar to the experimental group,transction ACL. Rats were sacrificed at 4,8 and 12 weeks after surgery,the synovial tissue wore obtain,and the expression of protein and gene of β-catenin was measured by immunohistochemistry（IHC） and semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）in different times of experimental groups and control groups. Results： Results from IHC showed that β- catenin was expressed in all experimental rats,and the expressions of β-catenin 4/8/12 weeks were different（all P 〈 0.05）. The expressions of β-catenin in 4 and 8 weeks were not different in control groups,and the expression of β-catenin was positive in part of the control group in 12 weeks. Semi-quantitative RT-PCR showed that the expression of β-catenin in the experimental group was higher than that of the control group ,and the expression increased with the extension of time in the experimental group, and the expression of β-catenin of control groups were different 4/8/12 weeks among（P 〈 0.05）. Conclusion： β-catenin was expressed in the synovial tissue of OA at a high level. And its level was increased with the extension of time,which indicates that. β-catenin plays a critical role in the pathogenesis and progression of osteoarthritis.

关 键 词：骨关节炎 Β连环蛋白 免疫组化 半定量RT-PCR 

分 类 号：R684.3[医药卫生—骨科学]