HPLC法测定杞菊地黄丸中莫诺苷、马钱苷和丹皮酚的含量  被引量:15

Determination of morroniside,loganin and paeonol in Qiju Dihuang pills by HPLC

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作  者:李润泽[1,2] 常增荣[2] 傅欣彤[2] 郭洪祝[2] 张英涛[1] 

机构地区:[1]北京大学药学院,北京100191 [2]北京市药品检验所,北京100035

出  处:《药物分析杂志》2015年第2期351-354,共4页Chinese Journal of Pharmaceutical Analysis

基  金:国家药品标准提高暨2015版药典科研项目

摘  要:目的:建立一种快速、准确和实用的HPLC方法,用于同时测定杞菊地黄丸中莫诺苷、马钱苷和丹皮酚的含量。方法:采用Phenomenex Gemini C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.3%磷酸水溶液为流动相梯度洗脱,流速1.0 mL·min^-1,柱温40℃,检测波长为240 nm(莫诺苷、马钱苷)和274 nm(丹皮酚)。结果:莫诺苷、马钱苷、丹皮酚质量浓度分别在2.242~44.84μg·mL^-1(r=0.999 9)、2.130~42.60μg·mL^-1(r=1.000)、5.068~101.4μg·mL^-1(r=1.000)范围内,与峰面积线性关系良好;平均回收率分别为96.2%(RSD=0.44%)、96.0%(RSD=0.21%)、103.0%(RSD=0.65%)。结论:本方法可作为杞菊地黄丸中莫诺苷、马钱苷和丹皮酚含量同时测定的方法,适用于杞菊地黄丸的质量控制。Objective: To establish a rapid,accurate and feasible HPLC method for simultaneous determination of morroniside,loganin and paeonol in Qiju Dihuang pills. Methods: The separation was carried out on a Phenomenex Gemini C18( 4. 6 mm × 250 mm,5 μm) column,the mobile phase consisting of 0. 3% phosphoric acid solution( B)-acetonirtile( A) at a flow rate of 1. 0 mL·min^-1,with the column temperature of 40 ℃,the detection wavelength at 240 nm for morroniside and loganin and 274 nm for paeonol. Results: The calibration curve was liner over the range of 2. 242-44. 84 μg·mL^-1( r = 0. 999 9) for morroniside,2. 130-42. 60 μg·mL^-1( r = 1. 000) for loganin and 5. 068-101. 4 μg·mL^-1( r = 1. 000) for paeonol. The average recovery of the three components was 96. 2%( RSD = 0. 44%),96. 0%( RSD = 0. 21%),and 103. 0%( RSD = 0. 65%),respectively. Conclusion: The method can be used for simultaneous determination of morroniside,loganin and paeonol in Qiju Dihuang pills,and it is applicative applicable for quality control of Qiju Dihuang pills.

关 键 词:中药成方制剂 杞菊地黄丸 莫诺苷 马钱苷 丹皮酚 高效液相色谱法 

分 类 号:R917[医药卫生—药物分析学]

 

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