双抗体夹心酶联免疫吸附试验检测血清HMGB-1水平在胃癌患者中的临床意义  

Double antibody sandwich enzyme-linked immunosorbent assay in detection of serum HMGB-1 levels of patients with gastric cancer

作　　者：郭强 李井野 况立革 

机构地区：[1]本溪市中心医院普外肿瘤二科,辽宁本溪117000

出　　处：《中国微生态学杂志》2015年第2期196-198,共3页Chinese Journal of Microecology

摘　　要：目的应用双抗体夹心酶联免疫吸附试验(ELl SA法),分析胃癌患者血清HMGB-1(高迁移率族蛋白B-1)水平的临床意义。方法使用ELISA法对98例胃癌患者血清HMGB-1水平进行检测,用电化学发光法对血清癌胚抗原(CEA)含量进行测定,并和40例胃良性病变者及40例正常者进行比较。结果胃癌组患者血清中HMGB-1与CEA水平均明显高于正常对照组与胃良性病变组(P<0.01)。和传统肿瘤标志物CEA对比,血清中HMGB-1检测在胃癌早期患者中有良好敏感性(70.6%)与准确性(74.0%),并具有统计学意义(P<0.05)。结论 HMGB-1和胃癌的发生发展密切相关,检测血清中HMGB-1的含量可以提高早期胃癌的诊断水平。Objective To analyze the relationship between serum HMGB-1 level and patients with gastric cancer by double-antibody sandwich enzyme-linked immunosorbent assay（ ELl SA France）. Methods ELISA method was used to detect the serum HMGB-1 levels of 98 patients with gastric cancer. Electricity chemiluminescence was used to measure the serum carcinoembryonic antigen（ CEA） levels. 40 cases of benign gastric lesions and 40 healthy subjects were used as the controls. Results The serum HMGB-1 and CEA levels of gastric cancer patients were significantly higher than those of the healthy control group and benign gastric lesions group（ P 0. 01）. And compared to conventional tumor marker CEA,the serum HMGB-1 detection shoed better sensitivity（ 70. 6%） and accuracy（ 74. 0%） in patients with early gastric cancer,with a statistically significant difference（ P 0. 05）.Conclusion HMGB-1 and produce closely related to the development of gastric cancer,detection of serum HMGB-1 levels could improve the diagnosis of early gastric cancer.

关 键 词：高迁移率族蛋白B-1 酶联免疫吸附试验 胃癌 

分 类 号：R446.1[医药卫生—诊断学]