基于磷光猝灭的比率式光学氧气探针  

Ratiometric optical oxygen sensor based on phosphorescence quenching

作　　者：王瑞芳[1,2] 高建峰[1] 陈鹏忠[2] 陈玉哲[2] 杨清正[2] 

机构地区：[1]中北大学理学院,太原030051 [2]中国科学院理化技术研究所、光化学转换与功能材料重点实验室,北京100190

出　　处：《科学通报》2015年第4期344-355,共12页Chinese Science Bulletin

基　　金：国家自然科学基金(21102155,21272243,21222210);国家重点基础研究发展计划(2013CB933800)资助

摘　　要：近几十年来,对氧气的有效检测和识别一直受到研究者的极大关注.传统的检测方法,如滴定法、电流分析法、热致发光法,存在着许多缺陷,如响应时间长、灵敏度差和检测过程中消耗氧气等,限制了其广泛应用.由于基态氧是三重态,能够猝灭三重态分子的发光,因此基于磷光强度变化的光学氧气探针克服了传统检测方法的不足,具有灵敏度高、选择性好、可逆性好、方便快捷以及无需消耗氧气等优点,逐渐成为研究热点.其中比率式的光学氧气探针同时引入对氧气不敏感的荧光分子作为内标和对氧气敏感的磷光分子作为指示剂分子,其输出信号依赖于内标分子和指示剂分子发光强度比例的变化,能够有效地避免环境和仪器的干扰,是检测氧气的理想模式.而且,大部分比率式氧气探针的溶液发光颜色会随氧气含量的变化而改变,从视觉上就可达到快速检测氧气的目的.本文从氧气检测机理、探针的构筑以及细胞成像等方面总结了近10年来比率式光学氧气探针的研究状况,并对其未来的发展做了一定的展望.Molecular oxygen plays a pivotal role in various biological, chemical and environmental reactions, thus detection of oxygen has attracted much attention. Traditional methods of sensing oxygen including classical Winkler titration, electroanalysis, chemiluminescence and thermoluminescence suffer from limitations such as relatively long response time, oxygen consumption during the sensing process and poor selectivity. The ground state of oxygen is a triplet state, so oxygen can quench the long-lived triplet phosphorescence of luminophores. Optical sensors to detect oxygen through phosphorescence have become a very active research field because of their good sensitivity and selectivity, full reversibility, simplicity, suitability for real-time measurements and minimal consumption of oxygen during measurements. Common detection modalities in optical oxygen sensing include phosphorescence intensity, ratiometric and lifetime measurements. Ratiometric intensity measurements at two different wavelengths allow more reliable detection than at a single wavelength because oxygen responses depend on the ratio of the oxygen sensor and reference luminescent signals. In addition, most ratiometric optical oxygen sensors exhibit a perceivable color change, which is useful for rapid visual sensing This review focuses on the mechanism of oxygen sensing, material designs for ratiometric sensors and cell imaging. The future development of oxygen sensors is also discussed.

关 键 词：比率式 光学探针 氧气 磷光 猝灭 

分 类 号：O657.3[理学—分析化学] TQ116.14[理学—化学]