舒尼替尼抑制小鼠骨髓来源树突状细胞表面PD-L1和PD-L2的表达  

Inhibition of sunitinib on the expressions of PD-L1 and PD-L2 of mouse bone marrow-derived dendritic cells

作　　者：赵飞龙[1] 麦海星[2] 董金凯[2] 李学超[2] 汤永永[3] 陈立军[1,2] 张斌[3] 

机构地区：[1]安徽医科大学解放军307医院临床学院泌尿外科,北京100071 [2]军事医学科学院附属医院泌尿外科,北京100071 [3]军事医学科学院附属医院造血干细胞移植科,北京100071

出　　处：《细胞与分子免疫学杂志》2015年第1期10-13,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：吴阶平医学基金(32067521203)

摘　　要：目的观察小鼠髓源性树突状细胞(DC)在舒尼替尼刺激下,其表面程序性死亡分子1配体1(PD-L1)和PD-L2的表达变化。方法取小鼠骨髓细胞,对照组加入二甲基亚砜,实验组分别加入(100、200、300)ng/m L舒尼替尼,刺激48 h,用流式细胞术检测DC表面PD-L1和PD-L2的表达水平。结果与对照组相比,实验组中成熟DC(m DC)和总DC(包括m DC和im DC)表面PD-L1的表达水平显著降低;表达PD-L1的未成熟DC(im DC)、m DC和DC百分比均显著降低,表达PD-L2的m DC百分比显著降低;表达PD-L2的DC百分比在100 ng/m L、300 ng/m L舒尼替尼组中显著降低。结论舒尼替尼可显著降低小鼠DC表面PD-L1、PD-L2的表达。Objective To observe the changes of programmed death-1 ligand 1 （PD-L1） and PD-L2 expressions on mouse bone marrow-derived dendritic ceils （DCs） stimulated by sunitinib. Methods DCs were randomly divided into four groups which were treated with sunitinib （100, 200, 300 ng/mL） and dimethylsulfoxide （DMSO）, respectively. After 48 hours, PD-L1 and PD-L2 expression levels were analyzed by flow cytometry. Results Compared with the control group, the expression of PD-L1 on mature DCs （mDCs） and all DCs[ including mature DCs and immature DCs （imDCs） ] was significantly down-regulated in sunitinib treatment groups. The PD-L1 expression percentages of imDCs, mDCs and DCs were significantly reduced in sunitinib treatment groups; the percentage of mDCs expressing PD-L2 also dropped in all treatment groups, and the percentage of DCs expressing PD-L2 decreased in 100 and 300 ng/mL sunitinib treatment groups. Conclusion Sunitinib can significantly reduce the expressions of PD-L1 and PD-L2 on mouse DCs.

关 键 词：舒尼替尼 树突状细胞 程序性死亡分子1配体1 程序性死亡分子1配体2 肾细胞癌 

分 类 号：R392.11[医药卫生—免疫学] R967[医药卫生—基础医学]