八正散单味中药及7味清热利湿药体外抗沙眼衣原体活性研究  

Impacts of Bazhengsan and seven kinds of Qingrelishi traditional medcine on the chlamydia trachomatis in vitro

作　　者：黄敏[1] 郑和平[2] 段行武[1] 薛耀华[2] 李建红[1] 

机构地区：[1]北京中医药大学东直门医院皮肤科,北京100700 [2]广东省皮肤性病防治中心,广州510000

出　　处：《中国麻风皮肤病杂志》2015年第1期15-17,共3页China Journal of Leprosy and Skin Diseases

摘　　要：目的:明确八正散中单味中药及黄柏等7种清热利湿中药的体外抗沙眼衣原体活性。方法:应用微量McCoy细胞培养法检测八正散中单味中药及黄柏等7种清热利湿药体外抗沙眼衣原体的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。结果:生大黄、黄柏在体外有较强的抗沙眼衣原体活性,两者MIC与MBC相同,分别为6.25 mg/mL和12.5 mg/mL。其余中药未检测出MIC值与MBC值,但栀子、瞿麦在最大无毒浓度(TD_0)时孔内沙眼衣原体包涵体数目明显减少,体积缩小。结论:生大黄、黄柏具有较强的体外抗沙眼衣原体活性,栀子、瞿麦有一定的体外抗沙眼衣原体活性。Objective： To determine the inhibitory impacts of the single traditional Chinese herb from Bazhengsan and seven kinds of Qingrelishi traditional Medcine on the Chlamydia trachomatis （Ct）. Methods： The minimal inhibitory concentration （MIC） and the minimal bactericidal concentration （MBC） of these medicines on Ct was detected by microculture technique of McCoy cell in vitro. Results： The inhibitory activities of Shengdahuang and Huangbai on Ct were obvious and the concentration of MIC and MBC of them was 6.25 mg/mL and 12.5 mg/mL. MIC and MBC of other medicines were not detected, but the number and size of Ct inclusions in Zhizi and Qumai groups became less in TD0 concentration. Conclusion： Shengdahuang, Huangbai, Zhizi and Qumai can affect the activity of Ct, especially Shengdahnang and Huangbai.

关 键 词：八正散 清热利湿药 沙眼衣原体 药物敏感性试验 

分 类 号：R285[医药卫生—中药学]