Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys  

作　　者：Yongchang Chen Yiqiang Cui Bin Shen Yuyu Niu Xiaoyang Zhao Lei Wang Jianying Wang Wei Li Qi Zhou Weizhi Ji Jiahao Sha Xingxu Huang 

机构地区：[1]Yunnan Key Laboratory of Primate Biomedical Research, Kunming, Yunnan 650500, China [2]State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing, Jiangsu 210029, China [3]MOE Key Laboratory of Model Animal for Disease Study, Model Animal Research Center of Nanjing University, National Resource Center for Mutant Mice, Nanjing, Jiangsu 210061, China [4]State Key Laboratory of Reproduetive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [5]Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan 650500, China [6]Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650224, China, [7]Shanghai Key Laboratory of Reproductive Medi- cine, Shanghai 200025, China

出　　处：《Cell Research》2015年第2期262-265,共4页细胞研究（英文版）

摘　　要：Dear Editor, Precise gene targeting in monkeys will substantially facilitate generation of human disease models [1, 2]. Although several transgenic monkey models have been successfully generated [3-6], most of past endeavors in gene targeting using primates had unfortunately failed. The usage of reprogrammable endonucleases, such as ZFN （zinc-finger nuclease）, TALEN （transcription activator-like effector nuclease） and CRISPR （clustered regularly interspaced short palindromic repeat）/Cas （CRISPR-associated） system, has opened new avenues for precise gene modification in monkeys [1, 2]. Among these, the CRISPR/Cas9 system has shown to be most efficacious in gene targeting based on its successful application in a number of animal species [7, 8].

关 键 词：转基因 RNA介导 猴子 收购 生殖 修改 疾病模型 灵长类动物 

分 类 号：Q78[生物学—分子生物学] Q943.2