Recent advances of DNA sequencing via nanopore-based technologies  

纳米孔DNA测序的最新研究进展(英文)

作　　者：郭秉元 曾涛 吴海臣 

机构地区：[1]Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences [2]National Center for Nanoscience and Technology of China

出　　处：《Science Bulletin》2015年第3期287-295,I0001,共10页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (21175135, 21375130, 21205119);the National Basic Research Program of China (2010CB933600);the 100 Talents Program of the Chinese Academy of Sciences

摘　　要：This review briefly summarizes recent progress in nanopore DNA sequencing from the beginning of 2012 to July 2014. Although partial successes have been achieved in different types of nanopores, biological pores such as α- hemolysin and MspA afford most promising results.纳米孔DNA测序是目前广受关注的第三代测序技术的代表,其发展日新月异,尤其在近3年取得了令人瞩目的成果.本文以纳米孔构建材料为分类基础,综述了各种纳米孔在基因测序方面的最新研究进展.其中涵盖的纳米孔包括:生物纳米孔、固态纳米孔以及其他新型纳米孔,如二维单层纳米孔、DNA折纸纳米孔和超短单壁碳纳米管纳米孔.总体而言,生物纳米孔取得的进展最为引人瞩目,金黄色葡萄球菌α-溶血毒素纳米孔与耻垢分枝杆菌毒素蛋白A纳米孔均已克服测序中固有的两大难题,即DNA穿越速度过快和单个碱基分辨率问题,进入实际应用开发阶段.相比之下,其余几类纳米孔还处于前期基础研究阶段,有待进一步发展.笔者相信,经过科研工作者的不懈努力,纳米孔技术一定能在未来的测序市场与个人医疗领域取得成功.

关 键 词：DNA sequencing. Nanopore α-Hemolysin MspA phi29 connector Solid-state nanopore Graphene SWCNT 

分 类 号：R440[医药卫生—诊断学]