P2X4受体拮抗剂5-BDBD对过敏性哮喘小鼠气道炎症的影响  

Effects of P2X4R antagonist 5-BDBD on airway inflammation of allergic asthma in mice

作　　者：陈洪霞[1] 冯筱茜 王亮[2] 夏青青[2] 宋印利[1] 倪秀芹[2] 

机构地区：[1]哈尔滨医科大学大庆校区病理学教研室,黑龙江大庆163319 [2]哈尔滨医科大学大庆校区解剖学教研室,黑龙江大庆163319

出　　处：《解剖科学进展》2015年第1期7-9,13,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(No.81200011);黑龙江省博士后基金(No.LBH-Z12213)

摘　　要：目的观察P2X4受体拮抗剂5-BDBD对过敏性哮喘小鼠气道炎症的影响。方法实验BALB/c小鼠分为正常对照组、哮喘组、5-BDBD组。卵蛋白致敏及气道激发制作过敏性哮喘模型。应用Western blot检测肺组织P2X4受体表达水平,HE染色观察肺组织病理学改变,ELISA检测支气管肺泡灌洗液(BALE)中IL-1β、TNF-α的表达水平。结果过敏性哮喘小鼠肺组织P2X4受体表达明显增高;给予5-BDBD处理后过敏性哮喘小鼠气道周围炎性浸润明显减轻,支气管肺泡灌洗液中IL-1β、TNF-α的含量较哮喘组相比也明显降低。结论嘌呤碱P2X4受体拮抗剂5-BDBD能够减轻过敏性哮喘小鼠气道炎症的发生。Objective To observe the effects of P2XgR antagonist, 5-BDBD, on airway inflammation of allergic asthma in mice. Methods BALB/c mice were sensitized and challenged with ovalbumin （OVA）. P2X4R level was determined by Western blot. Pathologic changes in the bronchi and lung tissues were examined by means of hematoxylin and eosin （HE） staining. Expression of IL-113 and TNF-α in bronchoalveolar lavage fluid （BALF） of mice were determined by ELISA. Results Compared with expression in control animals, P2X4R was overexpressed in the lungs after allergen challenge. Moreover, after the mice were treated with the P2X4R-specific antagonist 5-BDBD, the allergen-induced airway inflammation and levels of 1L-1β and TNF- α were decreased compared with those in asthma group. Condusion These results suggest that P2X4R antagonist, 5-BDBD, may attenuate the pathologic changes and airway inflammation in ovalbumin-sensitized

关 键 词：哮喘 5-BDBD 嘌呤碱P2X4受体 白介素-1Β 肿瘤坏死因子-α 

分 类 号：R562.25[医药卫生—呼吸系统]