玉郎伞水提物对心肌缺血再灌注损伤心肌组织ATP酶和凋亡蛋白的影响  

Effects of aqueous extracts of Yulangsan on ATPase and apoptosis-related protein in myocardial tissues with myocardial ischemia reperfusion injury

作　　者：吕纪华[1,2] 覃斐章[1] 梁杏梅[1] 禤霏霏 黄仁彬[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西中医药研究院,广西南宁530022

出　　处：《中国医院药学杂志》2015年第5期413-417,共5页Chinese Journal of Hospital Pharmacy

基　　金：广西科技基础条件平台建设(编号:12-97-20;10-108-19);广西自然科学基金重点项目(编号:2011GXNSFD018030);南宁市科技攻关与新产品试制(编号:201102084C);南宁市青秀区科学研究与技术开发计划(编号:2013S12);2011年度留学人员科技活动项目择优资助(编号:人发<2001>33号)

摘　　要：目的:研究玉郎伞水提物(WYLS)对大鼠离体心脏缺血再灌注(I/R)损伤心肌组织Na+-K+-ATP酶、Ca2+-ATP酶和凋亡蛋白的影响。方法:采用Langendorff离体心脏灌流法,停灌30min再灌30min建立大鼠心肌缺血再灌注损伤模型。观察WYLS对心肌组织Na+-K+-ATP酶、Ca2+-ATP酶活性的影响、心肌组织病理学变化、凋亡相关蛋白Bcl-2和Bax表达的影响。结果:与I/R组比较,WYLS高剂量组Na+-K+-ATP酶、Ca2+-ATP酶的活性明显增加(P<0.05或P<0.01),心肌受损程度明显减轻(P<0.05)。同时,WYLS高剂量组心肌Bax蛋白表达明显降低(P<0.01),Bcl-2蛋白表达无明显影响(P>0.05),但Bcl-2/Bax的比率明显增加(P<0.05)。结论:WYLS能减轻大鼠心肌I/R损伤,作用机制可能与减轻钙超载、抑制某些凋亡相关蛋白表达有关。OBJECTIVE To study the effects of aqueous extracts of Yulangsan（WYLS）on the activities of Na^＋-K^＋-ATPase and Ca^2＋-ATPase and the apoptosis-related proteins in myocardial tissues with myocardial ischemia-reperfusion（I/R）injury in isolated rat hearts.METHODS Langendorff-perfusion isolated rat hearts were prepared.Myocardial ischemia reperfusion injury was induced by 30 minutes of global ischemia followed by 30 minutes of reperfusion.The activities of Na^＋-K^＋-ATPase and Ca^2＋-ATPase in myocardial tissues were determined.The pathological changes of myocardium and the expressions of apoptosis-related proteins Bcl-2 and Bax were analyzed.RESULTS Compared with I/R group,the activities of Na^＋-K^＋-ATPase and Ca^2＋-ATPase significantly increased in high dose WYLS group（P〈0.05 or P〈0.01）.The severity of myocardial injury in high dose WYLS group evidently lessened（P〈0.01）.The expression of Bax significantly decreased（P〈0.01）and the ratio of Bcl-2/Bax significantly increased in high dose WYLS group（P〈0.05）.CONCLUSION WYLS can protect against myocardial ischemia reperfusion injury,the mechanism of which is its effects on reducing calcium overload and down regulating the expression of apoptosis-related protein.

关 键 词：玉郎伞 心肌缺血再灌注 NA^+-K^+-ATP酶 CA^2+-ATP酶 凋亡相关蛋白 

分 类 号：R285.5[医药卫生—中药学]