盐穗木HcDmc1基因的克隆和表达分析  

Clone and Expression Analysis of HcDmc1 Gene from Halostachys caspica

作　　者：杜驰[1] 张富春[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《西北植物学报》2015年第2期220-226,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家"973"计划前期研究专项(2012CB722204);新疆重点实验室专项资金(2014KL001)

摘　　要：根据盐穗木盐胁迫下响应的转录组测序结果,克隆获得盐穗木DNA损伤修复基因的cDNA序列,其开放阅读框1 035bp,编码344个氨基酸,命名为HcDmc1。保守结构域分析显示,该基因编码的蛋白具有RecA蛋白家族典型的保守结构域;统进化树分析显示HcDmc1为独立的分支;亚细胞定位于细胞质,为无信号肽、不跨膜的稳定亲水性蛋白。实时荧光定量PCR分析表明,盐穗木在100mmol/L NaCl胁迫7d后,同化枝中HcDmc1基因表达迅速上调并达到最大值,约为对照组的6.58倍;在700mmol/L NaCl胁迫14d后根中HcDmc1基因表达最高,约为对照的1.79倍。研究表明,HcDmc1基因表达受盐胁迫诱导。According to the transcriptome of Halostachys caspica under salt stress, we isolated an cDNA fragment from H. caspica. The obtained cDNA was named as disrupted meiotic cDNAof H. caspica. Se- quence analysis indicated that HcDmc1 contains an open reading frame （ORF） of 1 035 bp,which encodes 344 amino acids. Conserved domain analysis showed that HcDmcl has a conserved domain of RecA protein family. Phylogenetic tree analysis indicated that HcDmcl is an independent branch, which is an stable hy- drophilic proteins sub-cellularly cytoplasm. Real time quantitative PCR results showed that the expression of HcDmc1 gene in branch after 100 mmol/L NaCl salt stress for 7d was rapidly up-regulated and reached the highest after 700 mmol/L,which was 6.58-fold of the control. While in roots the expression of HcD- mc1 reached the highest level at 14 d and about 1.79-fold of the control. Base on the experimental results, the expression of HcDmc1 gene could be induced by salt stress.

关 键 词：盐穗木 HcDmc1基因 盐胁迫 基因表达 

分 类 号：Q785[生物学—分子生物学] Q786