睡菜离体快繁技术的研究  

Rapid Propagation of Menyanthes trifoliata L.in vitro

作　　者：赵峰[1] 胡若洋[1] 杜思乐[1] 石雪飞[1] 李学东[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《西北植物学报》2015年第2期410-415,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：首都师范大学实验室开放基金

摘　　要：以睡菜的幼嫩茎段为外植体,接种到附加不同浓度激素配比(6-BA/NAA)的MS培养基,诱导睡菜愈伤组织、芽及根的生长。研究发现,外植体在1.0mg/L 6-BA+0.1mg/L NAA+MS的培养基上培养10d,可观察到浅绿色的愈伤组织。愈伤组织转接到4.0mg/L 6-BA+0.3mg/L NAA+MS培养基上2周左右可生成芽。对带芽的愈伤组织再进行诱导生根进而形成完整再生植株,最适根诱导培养基为0.3mg/L 6-BA+1.0mg/L NAA+MS培养基。该实验采用植物离体快繁技术成功建立了睡菜再生体系,为睡菜种苗规模化奠定了技术基础。Using the young segments of Menyanthes trifoliata L.as explants transferred to MS medium with different concentration hormone（6-BA/NAA） can introduce the calli,buds and root growth.The study found that explants cultured on MS medium with 1.0 mg/L 6-BA and 0.1 mg/L NAA after 10 days can be observed in light green calli tissue.Calli transferred to MS added 4.0 mg/L 6-BA and 0.3 mg/L NAA for 2 weeks produce buds.Only the calli with buds could induce roots to form the complete regeneration plants.The most suitable root inducing medium is MS medium added 0.3 mg/L 6-BA and 1.0 mg/L NAA.Plant micropropagation technology successfully established regeneration system of M.trifoliata L.in this experiment,and it provides a new way for the large-scale production of M.trifoliata L.strains.

关 键 词：睡菜 愈伤组织 植株再生 

分 类 号：Q813.13[生物学—生物工程]