不同寄主植物上烟粉虱次生共生菌感染与性比相关性  

Relationships between infection with facultative symbionts and sex ratio of Bemisia tabaci on different host plants

作　　者：瞿钰峰[1] 李永腾[1,2] 刘向东[2] 方继朝[1] 郭慧芳[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014 [2]南京农业大学植物保护学院昆虫学系,南京210095

出　　处：《应用昆虫学报》2015年第1期89-95,共7页Chinese Journal of Applied Entomology

基　　金：江苏省支撑计划(BE2010342);江苏省农业科技自主创新资金项目(cx(12)3059)

摘　　要：【目的】烟粉虱Bemisia tabaci(Gennadius)体内次生共生菌感染受寄主植物的影响,一些共生菌会引起害虫的雌性化,明确田间不同寄主植物上害虫种群中共生菌与性比的相关性,可有利于进一步了解烟粉虱田间种群暴发机制。【方法】采集田间不同寄主植物上烟粉虱成虫,观察其性比,并对其中次生共生菌进行分子检测,分析共生菌携带率与性比相关性。【结果】江苏南京地区棉花、番茄、黄瓜和红薯4种寄主植物上烟粉虱次生共生菌Hamiltonella和Rickettsia感染均存在显著差异,其中Hamiltonella为优势共生菌,感染率依次为:棉花>黄瓜>番茄>红薯。寄主植物间Wolbachia和Cardinium的感染率均无显著差异。各寄主植物上烟粉虱雌性比均高于60%,其中黄瓜上高达75.6%,但不同寄主植物间无显著差异。进一步分析表明,Hamiltonella和Rickettsia感染率均与烟粉虱雌性比呈显著的二次多项式相关性。当Hamiltonella和Rickettsia感染率分别低于69%和5%时,随着感染率提高,烟粉虱雌性比上升,当感染率高于上述值时,则随着感染率增加,雌性比下降。【结论】棉花、番茄、黄瓜和红薯4种寄主植物上烟粉虱均表现出雌性化,但不同寄主植物间性比无差异,烟粉虱体内次生共生菌与性比存在相关性。[Objectives] Infection of the whitefly, Bemisia tabaci （Gennadius） by faeultative symbionts varies with host plant and some facultative symbionts can cause feminization of the host. Studies of the relationship between facultative symbiont infection and sex ratio in field populations of B. tabaci will be helpful to understand the breakout mechanism of this insect pest. [Methods] B. tabaci adults were collected from field populations from four species of host plants in Nanjing, China, and their sex ratios and frequency of symbiont infection determined. [Results] Hamiltonella defensa and Rickettsia infection in B. tabaci varied with host plant, including cotton, tomato, cucumber and sweet potato. Hamiltonella was the most common symbiont on all host plants, and frequency of infection of this symbiont was highest on cotton, followed, in descending order, by cucumber, tomato, and sweet potato. The frequency of Wolbachia and Carclinium infection was not affected by host plant. Females comprised 〉 60% of all four B. tabaci populations and there was no significant difference in the proportion of females among different host plants. Regression analysis indicates that both Hamiltonella and Rickettsiainfection are related to a female biased sex ratio, and quadratic polynomial regression models established an association between infection frequency and female ratio. When the infection rates of Hamiltonella and Rickettsia were lower than 69% and 5%, respectively, the proportion of females increased with infection rate. However, when symbiont infection rates were higher than those above, female ratios decreased with infection rates. [Conclusion] B. tabaci populations on cotton, tomato, cucumber and sweet potato are all female-biased, and sex ratios are not significantly different among different host plants. The infection frequencies of facultative symbionts is related to the population sex ratio.

关 键 词：烟粉虱 寄主植物 雌性比 Hamiltonella RICKETTSIA 

分 类 号：S433[农业科学—农业昆虫与害虫防治]