4种检测柠条种子活力技术的比较分析  

Comparative analysis of four techniques for evaluating seed viability of Caragana korshinskii Kom

作　　者：李加国[1] 李松林 韩梅 汪晓峰[1] 

机构地区：[1]北京林业大学林木育种国家工程实验室 [2]内蒙古赤峰市左旗十三敖包镇农牧业技术服务中心 [3]内蒙古鄂尔多斯市林业治沙科学研究所

出　　处：《北京林业大学学报》2015年第1期15-21,共7页Journal of Beijing Forestry University

基　　金："948"国家林业局引进项目(2011-4-54);林业公益性行业科研专项(201104024)

摘　　要：种子保存是当前植物种质资源保存的最有效、最安全的方式之一,快速、微量、准确检测种子库中种子活力的变化是当前植物种质设施保存技术研究的热点与重点。本文利用谷胱甘肽氧化还原电位(EGSSG/2GSH)、差示扫描量热分析(DSC)、电子自旋共振(EPR)和非损伤微检测(NMT)4种目前较为先进的方法,研究柠条种子在人工老化下的活力变化规律。结果表明,柠条种子在50℃、相对湿度(RH)为50%的老化条件下,其活力指数的下降速率要快于发芽率的下降速率;通过建立回归模型发现,在检测发芽率变化方面,4种检测方法的优越性依次为EPR>NMT>DSC>EGSSG/2GSH;在检测活力指数变化方面,4种检测方法的优越性依次为NMT>EGSSG/2GSH=EPR>DSC。综合对检测柠条种子发芽率和活力指数拟合度情况,以及对种子非损伤的性质,认为NMT最适合用来跟踪监测贮藏种子的活力。Seed preservation is one of the most effective and safest ways to retain sources of plant germplasm. How to check changes in seed viability rapidly and accurately at a micro-scale is a major issue in research facilities for preserving plant germplasm. With the GSH redox potential （Eosso/2osH ）, differential scanning calorimetry analysis （DSC）, electron spin resonance （EPR） and a non-invasive micro-test （NMT） , we studied the variation in the pattern of seed viability for Caragana korshinskii under artificial aging. The results revealed that the seed vigor index decreased more quickly than the germination percentage under the aging conditions of 50 ℃ and RH of 50%. From regression modeling we found that the precedence of four advanced technologies was EPR 〉 NMT 〉 DSC 〉 EGss/2ss in terms of the rate of germination. Based on the vigor index, the precedence was NMT 〉 EGSSG/2GSH = EPR 〉 DSC. NMT is most suitable for detecting seed vigor while integrating the fitness of seed germination and vigor index, as well as considering that these technologies are non-invasive to seeds.

关 键 词：柠条 谷胱甘肽氧化还原电位 玻璃态 分子流动性 非损伤微测 

分 类 号：S722.16[农业科学—林木遗传育种]