健脾通络解毒方对胃癌前病变患者胃黏膜COX-2、NF-κBp65及Bcl-2表达的影响  

Effect of Jianpi Tongluo Jiedu Recipe on Expression Levels of COX-2, NF-κBp65, and Bcl-2 in Gastric Mucosa of Patients with Precancerous Lesions of Gastric Cancer

作　　者：白宇宁[1] 张平[2] 李理[3] 王少丽[1] 姚乃礼[1] 张润顺[1] 刘震[1] 燕东[1] 朱昱翎[1] 马继征[1] 彭艳[1] 张玲[3] 曹戬 朱岩[2] 刘伟[4] 

机构地区：[1]中国中医科学院广安门医院脾胃病科,北京100053 [2]中国中医科学院望京医院病理科,北京100102 [3]中国中医科学院广安门医院病理科,北京100053 [4]首都医科大学基础医学院,北京100069

出　　处：《中国中西医结合杂志》2015年第2期167-173,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072804)

摘　　要：目的探讨健脾通络解毒方对胃癌前病变(precancerous lesions of gastric cancer,PLGC)患者胃黏膜COX-2、NF-κBp65、Bcl-2、Bax蛋白表达,COX-2、Bcl-2 mRNA水平及细胞凋亡指数(apoptotic index,AI)的影响。方法应用健脾通络解毒方随证加减治疗65例PLGC患者6个月,采用免疫组织化学法检测患者治疗前后COX-2、NF-κBp65、Bcl-2及Bax蛋白表达水平,采用RT-PCR法检测其中54例患者COX-2及Bcl-2mRNA水平,同时应用原位末端标记(TUNEL)荧光标记法检测65例患者治疗前后AI的变化。结果与治疗前比较,治疗后患者胃黏膜COX-2、NF-κBp65及Bcl-2蛋白阳性表达水平降低(均P<0.01),Bax蛋白阳性表达水平升高(P<0.05),COX-2及Bcl-2 mRNA表达水平降低(P<0.05,P<0.01),AI则增加(P<0.05)。结论健脾通络解毒方可能通过NF-κBp65/COX-2、COX-2/Bcl-2及NF-κBp65/Bcl-2等信号转导通路促进细胞凋亡,从而发挥对PLGC的影响作用。Objective To explore the effect of Jianpi Tongluo Jiedu Recipe （JTJR） on protein ex- pression levels of COX-2, NF-KBp65, Bcl-2, and Bax, mRNA expression levels of COX-2 and Bcl-2, and the apoptotic index （AI） in gastric mucosa of patients with precancerous lesions of gastric cancer （PL- GC）. Methods Totally 65 PLGC patients were recruited and treated by JTJR （modified by syndrome typing）, one dose per day for six successive months. Protein expression levels of COX-2, NF-KBp65, Bcl-2, and Bax were detected in 65 patients using immunohistochemical （IHC） assay before and after treatment. mRNA expression levels of COX-2 and Bcl-2 were detected in 54 patients using reverse transcription-polymerase chain reaction （RT-PCR）. Meanwhile, changes of AI was detected in 65 patients using TdT-mediated dUTP-biotin nick end labeling （TUNEL） fluorescence method. Results After treatment with JTJR, positive protein expression levels of COX-2, NF-KBp65, and Bcl-2 were obviously decreased in the gastric mucosa of PLGC patients （P 〈0.01 ）, but Bax positive protein expression was found to behigher （P 〈0.05）. At the same time mRNA expression levels of COX-2 and Bcl-2 were significantly lower after treatment than before treatment （P 〈0.05, P 〈0.01 ） ; AI also increased after treatment （P 〈0.05）. Conclusion JTJR could promote apoptosis possibly via NF-KBp65/COX-2, COX-2/Bcl-2, and NF-KBp65/ Bcl-2 signaling pathways, thereby affecting PLGC patients.

关 键 词：胃癌前病变 健脾通络解毒方 NF-κBp65/COX-2/Bcl-2 细胞凋亡 

分 类 号：R273[医药卫生—中西医结合]