冠心病痰浊证与气虚证的代谢组学研究  

Metabonomics Research on Coronary Heart Disease Patients of Phlegm Turbidity Syndrome and Qi Deficiency Syndrome

作　　者：程鹏[1] 陈泽奇[1] 王东生[1] 

机构地区：[1]中南大学湘雅医院中西医结合科,长沙410008

出　　处：《中国中西医结合杂志》2015年第2期193-197,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家科技部国际合作项目(No.2010DFA32370);科技部科技支撑计划(No.2009BAI80B04);国家自然科学基金资助项目(No.81173198);湖南省科技厅国际合作重点项目(No.2008WK3002)

摘　　要：目的运用代谢组学研究方法探讨冠心病痰浊证与气虚证的中医分型与其代谢产物之间的关系。方法选取冠心病患者65例,其中痰凝心脉证组37例,心气虚弱证组28例。应用气相色谱-质谱联用仪GC/MS(gas chromatograph-mass spectrometer-computer,GC/MS)测定两种中医证型的冠心病患者血清内源性代谢物,并分析其代谢谱之间的差异。结果共扫描100多个色谱峰,将得到的色谱图与质谱库进行匹配鉴定,最终得到准确定性的化合物46个。MCTree分析结果显示,痰浊证及气虚证患者可被有效区分开,鉴别这两种证型贡献值最大的化合物为丝氨酸,其次为缬氨酸、2羟基丙酸等。对两种证型代谢物量进行比较,发现痰浊证丝氨酸、2-羟基丙酸显著高于气虚证组(P<0.05)。结论冠心病中医证型在代谢组学方面的差异,为冠心病的中医证候研究提供了基础,提示代谢组学技术可能成为中医辨证分型的新研究手段。Objective To study the correlation between Chinese medical types of coronary heart disease （CHD） [i.e. phlegm turbidity syndrome （PTS） and qi deficiency syndrome （QDS）] and their metabolites. Methods Recruited were 65 CHD patients including 37 cases of PTS and 28 cases of QDS. Serum endogenous metabolites in the two syndrome types were determined by gas chromatograph-mass spectrometer-computer （C-C/MS）, and their differences between their metabolic profiles analyzed. Results More than 100 chromatographic peaks were totally scanned. Chromatograms obtained was matched with mass spectrum bank, and finally we got the category contribution value of 46 kinds of substances. Results of MCTree analysis showed patients of PTS and patients of QDS could be effectively distin- guished. Compounds contributing to identify the two syndromes were sequenced as serine, valine, 2 hy- droxy propionic acid. Comparison of metabolites showed contents of serine and 2 hydroxy propionic acid were higher in patients of PTS than in patients of QDS （P 〈0.05）. Conclusion The differences in the metabonomics of CHD TCM syndrome types could provide material bases for TCM syndrome differentiation of CHD, indicating that metabonomics technologies might become a new research method for TCM syndrome typing.

关 键 词：代谢组学 冠心病 痰浊证 气虚证 

分 类 号：R259[医药卫生—中西医结合]