三种不同尺寸生物玻璃球体修复兔股骨髁缺损的成骨能力和降解性能比较  

Comparison of bioactive glass spheres with three different sizes in repair of femoral condyle defects in rabbits

作　　者：高鹏[1] 王臻[1] 张浩强[1] 吴畅 刘云[3] 肖鑫[1] 

机构地区：[1]第四军医大学西京医院全军骨科研究所,西安710032 [2]解放军第四二二中心医院肿瘤科 [3]第四军医大学西京医院肿瘤科,西安710032

出　　处：《中华创伤骨科杂志》2015年第2期160-164,共5页Chinese Journal of Orthopaedic Trauma

基　　金：国家自然科学基金（31170914）

摘　　要：目的 比较3种不同尺寸生物玻璃球体修复兔股骨髁缺损的成骨能力和降解性能.方法 取成年新西兰大白兔24只,双侧股骨髁制造直径为0.6 cm、深为1.2cm的缺损,随机分为4组（每组6只12侧股骨）：A组植入直径为90 ～ 300 μm的生物玻璃球体,B组植入直径为301 ～ 500 μm的生物玻璃球体,C组植入直径为501 ～ 720 μm的生物玻璃球体,D组不植入任何材料.术后6、12周取材,计算各组的新生骨量及残余材料占骨缺损区的面积百分比,评价3种不同尺寸生物玻璃球体的骨缺损修复能力和降解性能. 结果 术后6周,A组、B组、C组可见新骨生成,D组基本无新骨形成;A组、B组、C组材料均出现部分降解,其中A组材料降解最多.术后12周,A组、B组、C组成骨量显著增加,均多于D组,A组的新生骨量占骨缺损区面积百分比（35.25％±1.52％）高于B组（22.42％±1.86％）和C组（21.26％±1.36％）,差异均有统计学意义（P＜0.05）,而B组与C组之间比较差异无统计学意义（P＞0.05）;A组残余材料占骨缺损区面积百分比（13.77％±0.47％）较B组（20.52％±0.82％）、C组（20.00％±1.08％）低,差异均有统计学意义（P＜0.05）,而B组与C组之间比较差异无统计学意义（P＞ 0.05）.结论 直径为90 ～ 300 μm的生物玻璃球体具有更好的成骨能力和降解性能,能有效修复腔隙性缺损.Objective To compare bioactive glass spheres with 3 different sizes in terms of their osteogenic potential and biodegradation property for repair of femoral condyle defects in rabbits.Methods Defects of O.6 cm in diameter and 1.2 cm in depth were created in the bilateral femoral condyles of 24 adult New Zealand white rabbits before the animals were randomly assigned into 4 even groups （n =6）.The 90 to 300 μm glass spheres were implanted in group A,301 to 500 μm spheres in group B,501 to 720 μm spheres in group C,and nothing in group D.Samples were harvested at 6 and 12 weeks after operation to measure new bone formation and the percentage of residual material in the area of bone defect.The osteogenic and biodegradation properties of the glass spheres were evaluated using quantitative histological analysis.Results At 6 weeks,new bone formation was observed in the implants in groups A,B and C but not in group D.Partial biodegradation was also observed in groups A,B and C.At 12 weeks,the amount of new bone obviously increased in groups A,B and C and all the 3 groups had greater new bone formation than group D.The amount of new bone in group A （35.25% ± 1.52%） was significantly greater than that in group B （22.42% ±1.86%） and in group C （21.26% ±1.36%） （P 〈0.05）,but there was no significant difference between groups B and C （P 〉 0.05）.The percentage of residual material in the area of bone defect in group A （13.77% ±0.47%） was significantly smaller than that in group B （20.52% ±0.82%） and in group C （20.00% ± 1.08%） （P 〈 O.05）,but there was no such a significant difference between groups B and C （P 〉 0.05）.Conclusion Since bioactive glass spheres of 90 to 300 μm demonstrate better osteogenic potential and biodegradation property than those with the other 2 sizes,they can be used to effectively repair lacuna bone defects.

关 键 词：骨移植 生物相容性材料 成骨细胞 生物降解 股骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程] R687.3[医药卫生—基础医学]