阿托伐他汀对急性心肌梗死大鼠内皮微颗粒及心肌细胞凋亡的影响  

Effects of atorvastatin on release of endothelial microparticles and myocardial apoptosis in rats with acute myocardial infarction

作　　者：李强[1] 郭壮波[1] 伍光颖 李超霞[2] 钟志勇 

机构地区：[1]暨南大学医学院第四附属医院,广州市红十字会医院急诊科,广东广州510220 [2]暨南大学医学院第四附属医院,广州市红十字会医院检验科,广东广州510220 [3]广东省医学动物实验中心,广东佛山528248

出　　处：《中国病理生理杂志》2015年第2期359-363,373,共6页Chinese Journal of Pathophysiology

基　　金：广东省科技计划(No.2012B061700016)

摘　　要：目的:探讨阿托伐他汀(atorvastatin,AT)对大鼠急性心肌梗死早期内皮微颗粒(endothial microparticles,EMP)及心肌细胞凋亡的影响。方法:将24只雄性SD大鼠随机分为假手术组(sham组)、心肌梗死组(MI组)和阿托伐他汀心肌梗死组(MI+AT组),每组8只。采用冠状动脉结扎制作急性心肌梗死大鼠模型。分别在造模后2 h和24 h采外周血检测肌酸激酶同工酶(creatine kinase-MB,CK-MB)、心肌肌钙蛋白T(cardiac troponin T,c Tn T)和EMP,其中循环EMP用流式细胞术检测。通过TUNEL检测心肌细胞凋亡情况。结果:造模后2 h,MI组大鼠的CK-MB表达水平较sham组大鼠显著升高(P<0.05);MI组及MI+AT组大鼠EMP表达水平及心肌细胞凋亡率上升,显著高于sham组(P<0.05)。造模后24 h,MI组大鼠EMP表达水平显著高于sham组(P<0.05);MI+AT组大鼠CK-MB、c Tn T、EMP表达水平及心肌细胞凋亡率较MI组显著降低(P<0.05)。此外,MI组大鼠的CKMB表达水平在造模后24 h较造模后2 h显著升高(P<0.05);MI+AT大鼠CK-MB、c Tn T和EMP表达水平在造模后24 h较2 h显著下降(P<0.05)。结论:AT可降低大鼠急性心肌梗死时的EMP水平和心肌细胞凋亡率,提示AT对内皮功能有保护作用。AIM： To investigate the effect of atorvastatin（ AT） on the release of endothelial microparticles（ EMP） and myocardial apoptosis in the rats with myocardial infarction. METHODS： SD male rats（ n = 24） were randomly divided into 3 groups： sham operation（ sham） group,myocardial infarction（ MI） group and MI ＋ AT group. The rat model of acute myocardial infarction was prepared by coronary artery ligation. At 2 h and 24 h after modeling,the peripheral blood was collected to detect creatine kinase-MB（ CK-MB） and cardiac troponin T（ c Tn T）. The circulating levels of EMP were measured by flow cytometry. The myocardial apoptosis was detected by terminal deoxynucleotidyl transferasemediated d UTP nick end labeling（ TUNEL） assay. RESULTS： At 2 h after modeling,the level of CK-MB was significantly increased in MI group compared with sham group,and the level of EMP and the myocardial apoptotic rate were significantly increased in MI group and MI ＋ AT group compared with sham group. At 24 h after modeling,the level of EMP was significantly increased in MI group compared with sham group. The levels of CK-MB,c Tn T,EMP and the myocardial apoptotic rate were significantly decreased in MI ＋ AT group compared with MI group. Moreover,the level of CK-MB in MI group was significantly increased at 24 h compared with that at 2 h after modeling. The levels of CK-MB,c Tn T and EMP were significantly decreased in MI ＋ AT group at 24 h compared with those at 2 h after modeling. CONCLUSION： Atorvastatin may reduce the level of EMP and the myocardial apoptotic rate in the rats with acute myocardial infarction,indicating that atorvastatin plays a role in protecting endothelium.

关 键 词：急性心肌梗死 阿托伐他汀 内皮微颗粒 心肌细胞凋亡 

分 类 号：R542.21[医药卫生—心血管疾病] R363[医药卫生—内科学]