膝骨关节炎严重程度与关节液和血清中chemerin含量的关系  

Correlation between the severity of knee osteoarthritis and levels of chemerin in serum and synovia

作　　者：段国庆[1] 任春凤[2] 

机构地区：[1]济宁医学院附属医院关节与运动医学科,山东省济宁市272029 [2]济宁市第一人民医院风湿免疫科,山东省济宁市272011

出　　处：《中国组织工程研究》2015年第2期177-181,共5页Chinese Journal of Tissue Engineering Research

基　　金：山东省医药卫生科技发展计划(2013ws0339);济宁市医药卫生科技发展计划(2013JNNK23)~~

摘　　要：背景:研究发现,chemerin受刺激后可进一步扩增炎症信号,导致炎症因子和基质金属蛋白酶的释放。提示chemerin可能作为软骨代谢的重要调控因子,在骨关节炎的病理过程中发挥重要的作用。目的:分析膝骨关节炎患者关节液和血清中chemerin水平与骨关节炎严重程度的相关性。方法:选择经临床确诊的膝骨关节炎患者80例,采用酶联免疫吸附试验(ELISA)法对关节液和血清中的chemerin水平进行检测,骨关节炎影像学严重程度评分采用国际公认的Kellgren-Lawrence(K-L)评分系统。结果与结论:患者关节液和血清中chemerin水平与Kellgren-Lawrence分级有显著相关性(r=0.981,P<0.001和r=0.901,P<0.001),且血清中chemerin水平与关节液中chemerin水平呈正相关(r=0.596,P<0.01)。结果表明,骨关节炎患者关节液和血清中chemerin水平与影像学K-L分级严重程度正相关,chemerin水平可作为骨关节炎诊断及判断严重程度的参考指标。BACKGROUND：Studies have found that after stimulation, chemerin can further amplify the inflammatory signal, resulting in the release of inflammatory factors and matrix metaloproteinases. It indicates that chemerin may serve as an important regulator of cartilage metabolism, and plays an important role in the pathogenesis of osteoarthritis. OBJECTIVE： To explore the correlation between level of chemerin in synovia and serum and severity of knee osteoarthritis. METHODS： Eighty patients with knee osteoarthritis were enroled in the study. Knee osteoarthritis grading was evaluated according to the Kelgren-Lawrence classification. Chemerin levels in synovia and serum were examined with enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION：The level of chemerin in synovia and serum in patients with knee osteoarthritis were positively correlated with the Kelgren-Lawrence classification （r=0.981,P〈 0.001;r=0.901,P 〈 0.001）. And there was a positive correlation between the level of chemerin in synovia and in serum （r=0.596,P 〈 0.01）. Experimental findings suggest that the levels of chemerin in synovial fluid and serum that are positively correlated with the Kelgren-Lawrence classification can be used as a reference biomarker of severity of knee osteoarthritis.

关 键 词：组织构建 骨组织工程 CHEMERIN 酶联免疫吸附试验 

分 类 号：R318[医药卫生—生物医学工程]