海螵蛸富血小板血浆液复合物修复兔软骨损伤的可行性  

Feasibility of cuttlebone/platelet-rich plasma compound in the repair of rabbit's cartilage injuries

作　　者：毕衡[1] 王清[1] 刘涛[1] 杨立丁[1] 姚哓 普宏润 叶国裕[1] 向俊宜[1] 

机构地区：[1]云南中医学院第一附属医院骨伤科,云南省昆明市650021

出　　处：《中国组织工程研究》2015年第3期352-357,共6页Chinese Journal of Tissue Engineering Research

基　　金：云南省自然科学基金立项课题(自筹)(2011FZ265)~~

摘　　要：背景:富血小板血浆是自体血小板的浓集物,富含大量生长因子,可以促进组织中细胞和基质的再生,促进组织修复,但其容易流动,不能有效固定于损伤部位。目的:以海螵蛸生物支架固定富血小板血浆,观察海螵蛸富血小板血浆复合物修复兔软骨损伤的效果。方法:将21只大耳白兔随机均分为3组,在兔右膝关节负重髁软骨面制作类似Hulth骨软骨损伤模型,实验组于骨损伤处植入海螵蛸支架-自体富血小板血浆复合物,对照组于骨缺损处植入明胶海绵-富血小板血浆复合物,空白对照组不植入任何材料。术后12周取材,进行大体观察与组织学观察。结果与结论:术后12周,实验组软骨缺损区域明显缩小,组织学观察缺损区域出现类软骨细胞,排列整齐,成柱状分布,非常接近正常软骨组织;对照组软骨缺损区域有轻度缩小,组织学观察骨基质表面可见类软骨细胞基质组织,但排列较为紊乱;空白对照组软骨缺损区域有轻度缩小,组织学观察到只有骨细胞及骨基质,血管组织存在,未见到软骨及类软骨组织。实验组缺损直径及深度均低于其他两组(P<0.05)。结果表明海螵蛸支架-富血小板血浆复合物可促进软骨缺损愈合。BACKGROUND：Platelet-rich plasma is a kind of autologous platelet concentrate that includes a large number of growth factors, and it can promote the regeneration of the tissue cels and matrix, and stimulate tissue repair, whereas it is easy to flow, and thus cannot be effectively fixed in the injured site. OBJECTIVE：To observe the effect of cuttlebone/platelet-rich plasma compound to repair the rabbit cartilage. METHODS：Twenty-one big-ear white rabbits were randomly divided into three groups. A model of osteochondral injury was made in the cartilage of the weight-bearing condyle of the rabbit right knee in the three groups, and respectively treated with implantation of cuttlebone/platelet-rich plasma compound in the experimental group, implantation of gelatin sponge/platelet-rich plasma compound in the control group, and nothing in the blank control group. The specimens were examined at 12 weeks postoperatively for gross observation and histological observation. RESULTS AND CONCLUSION：At 12 weeks after surgery, in the experimental group, the cartilage defect region was reduced significantly, chondrocyte-like cels appeared and arranged in alignment, showing a columnar distribution that is very close to the normal cartilage; in the control group, the cartilage defect region was narrowed a little, cartilage matrix-like tissues were visible but irregular; the blank control group, the cartilage＆amp;nbsp;defect region was narrowed slightly, and only bone cels, bone matrix and blood vessels were seen with no presence of cartilage or cartilage-like tissues. The defect diameter and depth in the experimental group were lower than those in the other two groups at 12 weeks after surgery （P 〈 0.05）. These findings suggest that the cuttlebone/platelet-rich plasma compound can promote the healing of cartilage defects.

关 键 词：生物材料 软骨生物材料 海螵蛸 富血小板血浆液 骨软骨 生物支架 云南省自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]